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Literature summary for 3.2.1.52 extracted from

  • Yamamoto, K.; Tsuji, Y.; Matsushita, S.; Kumagai, H.; Tochikura, T.
    Purification and properties of beta-N-acetylhexosaminidase from Mucor fragilis grown in bovine blood (1986), Appl. Environ. Microbiol., 51, 1019-1023.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ low molecular weight form EII, at 0.25 mM Mucor fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside low molecular weight form EII Mucor fragilis
0.46
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside low molecular weight form EII Mucor fragilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Mucor fragilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 low molecular weight form EII, SDS-PAGE Mucor fragilis
125000
-
 low molecular weight form EII, gel filtration Mucor fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Mucor fragilis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Mucor fragilis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein low molecular weight form EII Mucor fragilis

Purification (Commentary)

Purification (Comment) Organism
low molecular weight form EII, 3500fold to homogeneity Mucor fragilis

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate low molecular weight form Mucor fragilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.2
-
 low molecular weight form EII, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Mucor fragilis
35.2
-
 low molecular weight form EII, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Mucor fragilis

Storage Stability

Storage Stability Organism
-20°C, low molecular weight form EII, concentrated by ultrafiltration Mucor fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycopeptide + H2O low molecular weight form EII Mucor fragilis N-acetyl-D-glucosamine
-
 ?
glycopeptide + H2O glycopeptide obtained by modification of fetuin Mucor fragilis N-acetyl-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + H2O low molecular weight form EII Mucor fragilis N-acetyl-D-glucosamine
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Mucor fragilis N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O low molecular weight form EII Mucor fragilis 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O low molecular weight form EII Mucor fragilis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
tri-N-acetylchitotriose + H2O low molecular weight form EII Mucor fragilis N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, low molecular weight form EII Mucor fragilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 low molecular weight form EII, stable for 10 min Mucor fragilis
57.5
-
 low molecular weight form EII, 50% of activity remains after 10 min Mucor fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, low molecular weight form EII Mucor fragilis
5.5 6.5 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, low molecular weight form EII Mucor fragilis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 low molecular weight form EII Mucor fragilis