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Literature summary for 3.2.1.52 extracted from

  • Frohwein, Y.Z.; Gatt, S.
    Isolation of beta-N-acetylhexosaminidase, beta-N-acetylglucosaminidase, and beta-N-acetylgalactosaminidase from calf brain (1967), Biochemistry, 6, 2775-2782.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-ethylmaleimide N-acetylgalactosaminidase Rattus norvegicus
N-ethylmaleimide N-acetylgalactosaminidase Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acetate inhibits N-acetylhexosaminidase Bos taurus
acetate inhibits N-acetylhexosaminidase Rattus norvegicus
Ag+ inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Bos taurus
Ag+ inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Rattus norvegicus
galactosamine inhibits N-acetylhexosaminidase Bos taurus
galactosamine inhibits N-acetylhexosaminidase Rattus norvegicus
glucosamine inhibits N-acetylhexosaminidase Bos taurus
glucosamine inhibits N-acetylhexosaminidase Rattus norvegicus
N-acetyl-D-galactosamine inhibits N-acetylhexosaminidase and -galactosaminidase Bos taurus
N-acetyl-D-galactosamine inhibits N-acetylhexosaminidase and -galactosaminidase Rattus norvegicus
N-acetyl-D-glucosamine inhibits N-acetylhexosaminidase and -glucosaminidase Bos taurus
N-acetyl-D-glucosamine inhibits N-acetylhexosaminidase and -glucosaminidase Rattus norvegicus
N-ethylmaleimide N-acetylglucosaminidase Bos taurus
N-ethylmaleimide N-acetylglucosaminidase Rattus norvegicus
p-hydroxymercuribenzoate inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Bos taurus
p-hydroxymercuribenzoate inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylgalactosaminidase Rattus norvegicus
0.35
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylgalactosaminidase Bos taurus
0.54
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylhexosaminidase Rattus norvegicus
0.54
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylhexosaminidase Bos taurus
0.72
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylglucosaminidase Rattus norvegicus
0.72
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylglucosaminidase Bos taurus
0.8
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylhexosaminidase Rattus norvegicus
0.8
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylhexosaminidase Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Rattus norvegicus
-
N-acetyl-D-hexosamines
-
?
N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
N-acetylglucosaminidase and -galactosaminidase Rattus norvegicus
N-acetylhexosaminidase: 63fold Rattus norvegicus
N-acetylhexosaminidase: 63fold, N-acetylglucosaminidase and -galactosaminidase Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Rattus norvegicus
0.03
-
N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Bos taurus
0.22
-
N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Rattus norvegicus
0.22
-
N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bos taurus

Storage Stability

Storage Stability Organism
-20°C, N-acetyl-glucosaminidase and -galactosaminidase completely lose activity after several days Rattus norvegicus
-20°C, N-acetyl-glucosaminidase and -galactosaminidase completely lose activity after several days Bos taurus
-20°C, N-acetyl-hexosaminidase, in presence of ammonium sulfate, after several weeks: precipitate devoid of enzymatic activity forms but can be removed by centrifugation, further purification: loss of activity after several days at -20°C Rattus norvegicus
-20°C, N-acetyl-hexosaminidase, in presence of ammonium sulfate, after several weeks: precipitate devoid of enzymatic activity forms but can be removed by centrifugation, further purification: loss of activity after several days at -20°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitobiose + H2O
-
Rattus norvegicus 2 D-glucosamine
-
?
chitobiose + H2O
-
Bos taurus 2 D-glucosamine
-
?
lacto-N-triose + H2O
-
Rattus norvegicus ?
-
?
lacto-N-triose + H2O
-
Bos taurus ?
-
?
N-acetyl-beta-D-glucosaminide-1,4-muramic acid + H2O
-
Rattus norvegicus ?
-
?
N-acetyl-beta-D-glucosaminide-1,4-muramic acid + H2O
-
Bos taurus ?
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Rattus norvegicus N-acetyl-D-hexosamines
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Bos taurus N-acetyl-D-hexosamines
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Rattus norvegicus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Rattus norvegicus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
phenyl-N-acetyl-beta-D-glucosaminide + H2O
-
Rattus norvegicus phenol + N-acetyl-beta-D-glucosaminide
-
?
phenyl-N-acetyl-beta-D-glucosaminide + H2O
-
Bos taurus phenol + N-acetyl-beta-D-glucosaminide
-
?
trihexosylceramide + H2O
-
Rattus norvegicus ?
-
?
trihexosylceramide + H2O
-
Bos taurus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, N-acetylhexosaminidase Rattus norvegicus
3.8
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, N-acetylhexosaminidase Bos taurus
4.2
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, N-acetylhexosaminidase Rattus norvegicus
4.2
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, N-acetylhexosaminidase Bos taurus
5.2
-
N-acetylglucosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Rattus norvegicus
5.2
-
N-acetylglucosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bos taurus
5.5
-
N-acetylgalactosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Rattus norvegicus
5.5
-
N-acetylgalactosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Bos taurus