Literature summary for 3.2.1.46 extracted from

Dolcetta, D.; Perani, L.; Givogri, M.I.; Galbiati, F.; Orlacchio, A.; Martino, S.; Roncarolo, M.G.; Bongarzone, E.
Analysis of galactocerebrosidase activity in the mouse brain by a new histological staining method (2004), J. Neurosci. Res., 77, 462-464.

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Application

Application	Comment	Organism
medicine	economical and fast histochemical way to distinguish neural cells expressing galactocerebrosidase from thoses that are deficient. This method enables the assessment of enzyme activity in virally-transduced cells as well as the biodistribution of galactocerebrosidase activity in Twitcher mice under gene or cell therapy	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
neuron	hippocampal pyramidal neuron, cerebellar neuron	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	economical and fast histochemical way to distinguish neural cells expressing galactocerebrosidase from thoses that are deficient, using 5Br-3Cl-beta-galactopiranoside in the presence of taurodeoxycholic and oleic acids	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-bromo-3-chloro-beta-galactopyranoside + H2O	-	Mus musculus	?	-	?

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Release 2023.1 (January 2023)