Literature summary for 3.2.1.45 extracted from

Bobst, C.E.; Thomas, J.J.; Salinas, P.A.; Savickas, P.; Kaltashov, I.A.
Impact of oxidation on protein therapeutics: conformational dynamics of intact and oxidized acid-beta-glucocerebrosidase at near-physiological pH (2010), Protein Sci., 19, 2366-2378.

Cloned(Commentary)

Cloned (Comment)	Organism
gene-activated GCase is produced in an immortalized human cell line (HT-1080) utilizing targeted recombination with a promoter that activates the endogenous GCase in the presence of the mannosidase I inhibitor kifunensine, which results in a molecule with predominantly high-mannose-type glycans	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Oxidation Stability

Oxidation Stability	Organism
forced oxidation of GCase that results in a 40-60% reduction in in vitro biological activity affects the stability of some key structural elements within the catalytic site. These changes in dynamics occur on a longer time scale that is irrelevant for catalysis, effectively ruling out loss of structure in the catalytic site as a major factor contributing to the reduction of the catalytic activity. Oxidation also leads to noticeable destabilization of conformation in remote protein segments on a much larger scale, which is likely to increase the aggregation propensity of GCase and affect its bioavailability	Homo sapiens

Organism

forced oxidation of GCase that results in a 40-60% reduction in in vitro biological activity affects the stability of some key structural elements within the catalytic site. These changes in dynamics occur on a longer time scale that is irrelevant for catalysis, effectively ruling out loss of structure in the catalytic site as a major factor contributing to the reduction of the catalytic activity. Oxidation also leads to noticeable destabilization of conformation in remote protein segments on a much larger scale, which is likely to increase the aggregation propensity of GCase and affect its bioavailability

Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Homo sapiens	4-nitrophenol + D-glucopyranose	-	?

Synonyms

Synonyms	Comment	Organism
beta-glucocerebrosidase	-	Homo sapiens
Gcase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Homo sapiens