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Literature summary for 3.2.1.45 extracted from

  • Fabrega, S.; Durand, P.; Codogno, P.; Bauvy, C.; Delomenie, C.; Henrissat, B.; Martin, B.M.; McKinney, C.; Ginns, E.I.; Mornon, J.P.; Lehn, P.
    Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells (2000), Glycobiology, 10, 1217-1224.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant and wild-type enzymes in murine cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E235A the mutant is catalytically inactive Homo sapiens
E340A the mutant is catalytically inactive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
4-methylumbelliferyl-beta-D-glucopyranoside pH 5.9 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00007
-
mutant E235A Homo sapiens
0.00008
-
mutant E340A Homo sapiens
0.0046
-
wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
Homo sapiens 4-methylumbelliferone + beta-D-glucose
-
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