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Literature summary for 3.2.1.4 extracted from

  • Ito, Y.; Ikeuchi, A.; Imamura, C.
    Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library (2013), Protein Eng. Des. Sel., 26, 73-79 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
A241E improved thermal stability Phanerodontia chrysosporium
F206Y improved thermal stability Phanerodontia chrysosporium
M259I improved thermal stability Phanerodontia chrysosporium
M259L improved thermal stability Phanerodontia chrysosporium
N125K improved thermal stability Phanerodontia chrysosporium
Q204K improved thermal stability Phanerodontia chrysosporium
Q307R/A309S improved thermal stability Phanerodontia chrysosporium
Q381S/F382L improved thermal stability Phanerodontia chrysosporium
S183T improved thermal stability Phanerodontia chrysosporium
S22P thermostability similar to wild-type Phanerodontia chrysosporium
T112S improved thermal stability Phanerodontia chrysosporium
T130I/S134Q improved thermal stability Phanerodontia chrysosporium
T130I/S134Q/M259I/A241E/S183T/Q307R/A309S temperature required to reduce the initial activity by 50% within 120 min is increased by 4.4 degrees compared to wild-type Phanerodontia chrysosporium
T130I/S134Q/M259I/A241E/S183T/Q307R/A309S/T112S/N125K/Q204K/F206Y/I365 temperature required to reduce the initial activity by 50% within 120 min is increased by 5.4 degrees compared to wild-type, specific activity is the same as wild-type Phanerodontia chrysosporium
T342HI365V improved thermal stability Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q02321
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-

Synonyms

Synonyms Comment Organism
CBHII
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 Phanerodontia chrysosporium