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Literature summary for 3.2.1.4 extracted from

  • Moloney, A. P.; McCrae, S. I.; Wood, T. M.; Coughlan, M. P.
    Isolation and characterization of 1,4-beta-D-glucan glucanohydrolases of Talaromyces emersonii (1985), Biochem. J., 225, 365-374.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
additional information glucose (2 mM), cellobiose (1.25 mM), methylcellulose (0.5%, w/v) and gluconolactone (100 mM) do not inhibit the hydrolysis of carboxymethylcellulose by any of the endocellulases Rasamsonia emersonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE, endocellulases I, endocellulases II, endocellulases III, endocellulases IV Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content: endocellulases I (27.7%), endocellulases II (29.9%), endocellulases III (44.7%), endocellulases IV (50.8%) Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
 Rasamsonia emersonii ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
endocellulases I
-
 Rasamsonia emersonii
endocellulases II
-
 Rasamsonia emersonii
endocellulases III
-
 Rasamsonia emersonii
endocellulases IV
-
 Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 80 endocellulases I, endocellulases II, endocellulases III, endocellulases IV, substrate: carboxymethyl cellulose, pH 5.0 Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 half-life: endocellulases I (104 min), endocellulases II (93 min), endocellulases III (75 min), endocellulases IV (66 min) Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 5.8 endocellulases I, endocellulases II, endocellulases III, endocellulases IV, substrate: carboxymethyl cellulose, 60°C Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii isoelectric focusing, endocellulases IV
-
 2.86
Rasamsonia emersonii isoelectric focusing, endocellulases II
-
 3.08
Rasamsonia emersonii isoelectric focusing, endocellulases I
-
 3.19