Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Humicola insolens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 86% residual activity | Humicola insolens | |
cetyltrimethylammonium bromide | 1 mM, 31% residual activity | Humicola insolens | |
EDTA | 1 mM, 91% residual activity | Humicola insolens | |
Mn2+ | 1 mM, 59% residual activity | Humicola insolens | |
sodium dodecylsulfate | 1 mM, 76% residual activity | Humicola insolens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Barley beta-glucan | Km-value 1.29 mg/ml, pH 6.5, 70°C | Humicola insolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola insolens | A0A0F7Q1T8 | - |
- |
Humicola insolens Y1 | A0A0F7Q1T8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
372 | - |
substrate barley beta-glucan, pH 6.5, 70°C | Humicola insolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Humicola insolens | ? | - |
? | |
barley beta-glucan + H2O | - |
Humicola insolens Y1 | ? | - |
? | |
carboxymethyl cellulose + H2O | 13.6% of the activity with barley beta-glucan | Humicola insolens | ? | - |
? | |
carboxymethyl cellulose + H2O | 13.6% of the activity with barley beta-glucan | Humicola insolens Y1 | ? | - |
? | |
lichenan + H2O | 60.5% of the activity with barley beta-glucan | Humicola insolens | ? | - |
? | |
lichenan + H2O | 60.5% of the activity with barley beta-glucan | Humicola insolens Y1 | ? | - |
? | |
additional information | enzyme is strictly specific for the beta-1,4-glucoside linkage exhibiting activity toward barley beta-glucan, lichenan, and carboxymethyl cellulose sodium salt, but not toward laminarin (1,3-beta-glucan), birchwood xylan, avicel, and not toward 4-nitrophenyl beta-D-glucoside or 4-nitrophenyl cellobioside. Cel6C cleaves the internal glycosidic linkages of cellooligosaccharides randomly, the predominant product of polysaccharide hydrolysis is cellobiose | Humicola insolens | ? | - |
? | |
additional information | enzyme is strictly specific for the beta-1,4-glucoside linkage exhibiting activity toward barley beta-glucan, lichenan, and carboxymethyl cellulose sodium salt, but not toward laminarin (1,3-beta-glucan), birchwood xylan, avicel, and not toward 4-nitrophenyl beta-D-glucoside or 4-nitrophenyl cellobioside. Cel6C cleaves the internal glycosidic linkages of cellooligosaccharides randomly, the predominant product of polysaccharide hydrolysis is cellobiose | Humicola insolens Y1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel6C | - |
Humicola insolens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Humicola insolens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to | Humicola insolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Humicola insolens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
98% of maximum activity | Humicola insolens |
9 | - |
61% of maximum activity | Humicola insolens |
10 | - |
27% of maximum activity | Humicola insolens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | good stability | Humicola insolens |