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Literature summary for 3.2.1.4 extracted from

  • Yu, H.; Li, X.
    Alkali-stable cellulase from a halophilic isolate, Gracilibacillus sp. SK1 and its application in lignocellulosic saccharification for ethanol production (2015), Biomass Bioenergy, 81, 19-25.
No PubMed abstract available
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 5 mM, 82.3% residual activity, 50%, 48 h, crude enzyme preparation Gracilibacillus sp.
EDTA 5 mM, 17.9% residual activity, 50%, 48 h, crude enzyme preparation Gracilibacillus sp.
Hg2+ 5 mM, 21.9% residual activity, 50%, 48 h, crude enzyme preparation Gracilibacillus sp.
Mn2+ 5 mM, 82.5% residual activity, 50%, 48 h, crude enzyme preparation Gracilibacillus sp.
PMSF 5 mM, 10.1% residual activity, 50%, 48 h, crude enzyme preparation Gracilibacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Gracilibacillus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl more than 60% activity is retained after incubation with 0-25% NaCl for 48 h Gracilibacillus sp.

Organic Solvent Stability

Organic Solvent Comment Organism
1-butanol 50%, 48 h, crude enzyme preparation, 58.3% residual activity Gracilibacillus sp.
Acetone 50%, 48 h, crude enzyme preparation, 57.6% residual activity Gracilibacillus sp.
acetonitrile 50%, 48 h, crude enzyme preparation, 31.2% residual activity Gracilibacillus sp.
DMSO 50%, 48 h, crude enzyme preparation, 41% residual activity Gracilibacillus sp.
Ethanol 50%, 48 h, crude enzyme preparation, 25.1% residual activity Gracilibacillus sp.
hexane 50%, 48 h, crude enzyme preparation, 117.1% residual activity Gracilibacillus sp.
Methanol 50%, 48 h, crude enzyme preparation, 31.5% residual activity Gracilibacillus sp.

Organism

Organism UniProt Comment Textmining
Gracilibacillus sp.
-
-
-
Gracilibacillus sp. SK1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
218.9
-
 substrate cellobiose, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.
234.6
-
 substrate cotton straw, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.
257.8
-
 substrate wheat straw, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.
315.2
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.
354.1
-
 substrate rice straw, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.
429.7
-
 substrate corn stover, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.
510.8
-
 substrate carboxymethyl cellulose, crude enzyme preparation, pH 8.0, 60°C Gracilibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Gracilibacillus sp. 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Gracilibacillus sp. SK1 4-nitrophenol + D-glucose
-
 ?
carboxymethyl cellulose + H2O
-
 Gracilibacillus sp. ?
-
 ?
carboxymethyl cellulose + H2O
-
 Gracilibacillus sp. SK1 ?
-
 ?
cellobiose + H2O
-
 Gracilibacillus sp. 2 D-glucose
-
 ?
cellobiose + H2O
-
 Gracilibacillus sp. SK1 2 D-glucose
-
 ?
corn stover + H2O
-
 Gracilibacillus sp. ?
-
 ?
cotton straw + H2O
-
 Gracilibacillus sp. ?
-
 ?
rice straw + H2O
-
 Gracilibacillus sp. ?
-
 ?
rice straw + H2O
-
 Gracilibacillus sp. SK1 ?
-
 ?
wheat straw + H2O
-
 Gracilibacillus sp. ?
-
 ?
wheat straw + H2O
-
 Gracilibacillus sp. SK1 ?
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 48 h, complete loss of activity, crude cellulase preparation Gracilibacillus sp.
7 9 48 h, high stability, crude cellulase preparation Gracilibacillus sp.

Expression

Organism Comment Expression
Gracilibacillus sp. extracellular cellulase is expressed from the early-exponential phase (4 h) and reaches a maximum level during the stationary phase of bacterial growth (20 h) additional information
Gracilibacillus sp. expression is induced in presence of carboxymethyl cellulose up

General Information

General Information Comment Organism
physiological function Gracilibacillus sp. secretes six different endoglucanases Gracilibacillus sp.