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Literature summary for 3.2.1.4 extracted from

  • Rawat, R.; Kumar, S.; Chadha, B.S.; Kumar, D.; Oberoi, H.S.
    An acidothermophilic functionally active novel GH12 family endoglucanase from Aspergillus niger HO: purification, characterization and molecular interaction studies (2015), Antonie Van Leeuwenhoek, 107, 103-117.
    View publication on PubMed

Application

Application Comment Organism
degradation hydrolysis of 2% carboxymethyl cellulose with purified enzyme at its optimum temperature and pH results in complete hydrolysis within 2 h yielding 18% cellotriose, 72% cellobiose and 10% glucose Aspergillus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking studies reveal Glu116 and Glu204 as important for functional interaction with carboxymethylcellulose and show hydrogen bonding with Asp99, Glu116, Glu204 and hydrophobic interactions with Trp22, Val58, Tyr61, Phe101, Met118, Trp120, Pro129, Ile130, Thr160 and Phe206 Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Aspergillus niger
Hg2+ strong inhibition Aspergillus niger
Mn2+ strong inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
carboxymethyl cellulose Km-value is 2.5 mg/ml, pH 3.5, 70°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation Aspergillus niger
Co2+ stimulation Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger O74705
-
-
Aspergillus niger HO O74705
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.5
-
pH 3.5, 70°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucoside + H2O
-
Aspergillus niger 4-nitrophenol + beta-D-glucose 14% of the activity with carboxymethyl cellulose ?
4-nitrophenyl beta-D-glucoside + H2O
-
Aspergillus niger HO 4-nitrophenol + beta-D-glucose 14% of the activity with carboxymethyl cellulose ?
carboxymethyl cellulose + H2O
-
Aspergillus niger cellobiose + cellotriose + ? products are 18% cellotriose, 72% cellobiose and 10% glucose ?
cellobiose + H2O
-
Aspergillus niger ? 9.8% of the activity with carboxymethyl cellulose ?
cellobiose + H2O
-
Aspergillus niger HO ? 9.8% of the activity with carboxymethyl cellulose ?
filter paper + H2O
-
Aspergillus niger ? 18% of the activity with carboxymethyl cellulose ?
additional information no substrate: cellulose Aspergillus niger ?
-
?
additional information no substrate: cellulose Aspergillus niger HO ?
-
?
xylan + H2O
-
Aspergillus niger ? 20% of the activity with carboxymethyl cellulose ?
xylan + H2O
-
Aspergillus niger HO ? 20% of the activity with carboxymethyl cellulose ?
xyloglucan + H2O
-
Aspergillus niger ? 71% of the activity with carboxymethyl cellulose ?
xyloglucan + H2O
-
Aspergillus niger HO ? 71% of the activity with carboxymethyl cellulose ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
EglA
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life 3 h Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
2
-
about 75% of maximum activity Aspergillus niger
8
-
about 10% of maximum activity Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
3 h, no loss of activity Aspergillus niger