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Literature summary for 3.2.1.4 extracted from

  • Xia, D.; Wei, Y.; Zhang, G.; Zhao, Q.; Zhang, Y.; Xiang, Z.; Lu, C.
    cDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi (2013), Gene, 514, 62-68.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in baculovirus-infected insect BmN cells and Bombyx mori larvae Batocera horsfieldi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively Batocera horsfieldi
33000
-
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively Batocera horsfieldi

Organism

Organism UniProt Comment Textmining
Batocera horsfieldi
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein protein sequence has three putative N-glycosylation sites at residues 56-58, 99-101, and 237-239, respectively. Gylcosylated protein shows a molecular mass of 33 kDa compared with 28 kDa for ungycosylated form Batocera horsfieldi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
928
-
pH 6.0, 50°C Batocera horsfieldi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Batocera horsfieldi ?
-
?

Subunits

Subunits Comment Organism
? x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively Batocera horsfieldi

Synonyms

Synonyms Comment Organism
EGase II
-
Batocera horsfieldi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Batocera horsfieldi

pH Range

pH Minimum pH Maximum Comment Organism
6
-
-
Batocera horsfieldi

pI Value

Organism Comment pI Value Maximum pI Value
Batocera horsfieldi calculated
-
7.6