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Literature summary for 3.2.1.4 extracted from

  • Li, X.; Yu, H.Y.
    Halostable cellulase with organic solvent tolerance from Haloarcula sp. LLSG7 and its application in bioethanol fermentation using agricultural wastes (2013), J. Ind. Microbiol. Biotechnol., 40, 1357-1365.
    View publication on PubMed

Application

Application Comment Organism
biofuel production bioethanol fermentation using agricultural wastes Haloarcula sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Haloarcula sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl no activity in absence of NaCl, optimal activity of crude enzyme at 20% NaCl Haloarcula sp.

Organic Solvent Stability

Organic Solvent Comment Organism
1-butanol 25% (v/v), 30°C, 10 min, 56.1% residual activity Haloarcula sp.
1-decanol 25% (v/v), 30°C, 10 min, 81.8% residual activity Haloarcula sp.
Acetone 25% (v/v), 30°C, 10 min, 47.3% residual activity Haloarcula sp.
acetonitrile 25% (v/v), 30°C, 10 min, 51.8% residual activity Haloarcula sp.
benzene 25% (v/v), 30°C, 10 min, 127.9% residual activity Haloarcula sp.
chloroform 25% (v/v), 30°C, 10 min, 72.5% residual activity Haloarcula sp.
cyclohexane 25% (v/v), 30°C, 10 min, 75.0% residual activity Haloarcula sp.
dimethyl formamide 25% (v/v), 30°C, 10 min, 58.1% residual activity Haloarcula sp.
DMSO 25% (v/v), 30°C, 10 min, 41.2% residual activity Haloarcula sp.
Ethanol 25% (v/v), 30°C, 10 min, 35.2% residual activity Haloarcula sp.
isooctane 25% (v/v), 30°C, 10 min, 79.9% residual activity Haloarcula sp.
Methanol 25% (v/v), 30°C, 10 min, 38.1% residual activity Haloarcula sp.
n-hexane 25% (v/v), 30°C, 10 min, 126.1% residual activity Haloarcula sp.
toluene 25% (v/v), 30°C, 10 min, 84.1% residual activity Haloarcula sp.

Organism

Organism UniProt Comment Textmining
Haloarcula sp.
-
-
-
Haloarcula sp. LLSG7
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O maximal activity soluble cellulosic substrate (CMC-Na salt), indicating that the crude enzyme has endoglucanase activity Haloarcula sp. ?
-
?
cellulose + H2O maximal activity soluble cellulosic substrate (CMC-Na salt), indicating that the crude enzyme has endoglucanase activity Haloarcula sp. LLSG7 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
crude enzyme Haloarcula sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 40% of maximal activity, 80°C: about 70% of maximal activity, crude enzyme Haloarcula sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
crude enzyme Haloarcula sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 11 pH 7.0: about 85% of maximal activity, pH 11.0: about 70% of maximal activity Haloarcula sp.