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Literature summary for 3.2.1.4 extracted from

  • Kim, H.W.; Ishikawa, K.
    Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp. (2010), J. Microbiol. Biotechnol., 20, 889-892.
    View publication on PubMed
Search for more literature for 3.2.1.4

Application

Application Comment Organism
synthesis glucose production from cellulose material using beta-glucosidase from Pyrococcus furioses and endocellulase from Pyrococcus horikoshii. The combination reaction can produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
cellobiose the non-complete saccharification of cellulose by thr enzyme seems to be due to product-feedback inhibition by cellobiose Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58925
-
-
Pyrococcus horikoshii DSM 12428 O58925
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl cellobioside + H2O
-
 Pyrococcus horikoshii 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl cellobioside + H2O
-
 Pyrococcus horikoshii DSM 12428 4-nitrophenol + cellobiose
-
 ?
avicel + H2O the main product is cellobiose Pyrococcus horikoshii cellobiose + ?
-
 ?
avicel + H2O the main product is cellobiose Pyrococcus horikoshii DSM 12428 cellobiose + ?
-
 ?

Synonyms

Synonyms Comment Organism
EGPh
-
 Pyrococcus horikoshii
PH1171 locus name Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Pyrococcus horikoshii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
9.08
-
 cellobiose pH 5.5, 50°C Pyrococcus horikoshii