Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Graham, J.E.; Clark, M.E.; Nadler, D.C.; Huffer, S.; Chokhawala, H.A.; Rowland, S.E.; Blanch, H.W.; Clark, D.S.; Robb, F.T.
    Identification and characterization of a multidomain hyperthermophilic cellulase from an archaeal enrichment (2011), Nat. Commun., 2, 375.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression in Escherichia coli BL21 (DE3) and Rosetta cells Desulfurococcaceae archaeon EBI-244

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 x * 94000, SDS-PAGE Desulfurococcaceae archaeon EBI-244

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Desulfurococcaceae archaeon EBI-244 EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside ?
-
 ?

Organism

Organism UniProt Comment Textmining
Desulfurococcaceae archaeon EBI-244 F6M085 isolated from a sample of a circumneutral geothermal pool at 94 °C
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl cellobioside + H2O
-
 Desulfurococcaceae archaeon EBI-244 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Desulfurococcaceae archaeon EBI-244 ?
-
 ?
additional information EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside Desulfurococcaceae archaeon EBI-244 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 94000, SDS-PAGE Desulfurococcaceae archaeon EBI-244
More a multidomain cellulase Desulfurococcaceae archaeon EBI-244

Synonyms

Synonyms Comment Organism
More the enzyme is a a member of the TIM barrel glycosyl hydrolase superfamily (beta/alpha)8 Desulfurococcaceae archaeon EBI-244

General Information

General Information Comment Organism
additional information EBI-244 is a multidomain cellulase from a hyperthermophilic archaeon Desulfurococcaceae archaeon EBI-244