Crystallization (Comment) | Organism |
---|---|
purified catalytic core domain, sitting drop vapour diffusion technique, 0.001 ml of 8 mg/ml protein in 50 mM Tris-HCl, pH 7.0, are mixed with 0.001 ml of precipitation solution containing 35% PEG 4000, X-ray diffraction structure determination and analysis at 2.9-3.0 A resolution | Trichoderma harzianum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma harzianum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000, SDS-PAGE | Trichoderma harzianum |
50000 | - |
gel filtration | Trichoderma harzianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from fermentation medium by anion exchange chromatography, ultrafiltration and hydrophobic interaction chromatography followed by cleavage through papain to isolate the catalytic core domain, and gel filtration | Trichoderma harzianum |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Trichoderma harzianum |
Synonyms | Comment | Organism |
---|---|---|
CBHI | - |
Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Trichoderma harzianum |