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Literature summary for 3.2.1.4 extracted from

  • Colussi, F.; Textor, L.C.; Serpa, V.; Maeda, R.N.; Pereira, N.; Polikarpov, I.
    Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum (2010), Acta Crystallogr. Sect. F, 66, 1041-1044.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified catalytic core domain, sitting drop vapour diffusion technique, 0.001 ml of 8 mg/ml protein in 50 mM Tris-HCl, pH 7.0, are mixed with 0.001 ml of precipitation solution containing 35% PEG 4000, X-ray diffraction structure determination and analysis at 2.9-3.0 A resolution Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trichoderma harzianum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
1 * 46000, SDS-PAGE Trichoderma harzianum
50000
-
gel filtration Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from fermentation medium by anion exchange chromatography, ultrafiltration and hydrophobic interaction chromatography followed by cleavage through papain to isolate the catalytic core domain, and gel filtration Trichoderma harzianum

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
CBHI
-
Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Trichoderma harzianum