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Literature summary for 3.2.1.4 extracted from

  • Deng, Y.; Fong, S.S.
    Influence of culture aeration on the cellulase activity of Thermobifida fusca (2010), Appl. Microbiol. Biotechnol., 85, 965-974.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes Tfu_1627, Tfu_1074, Tfu_2176, and Tfu_0901, encoding endo-1,4-beta-glucanases, expression analysis during stationary and exponential growth, overview Thermobifida fusca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermobifida fusca
-
-

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
a filamentous bacterium, strain ATCC BAA-629, genes Tfu_1627, Tfu_1074, Tfu_2176, and Tfu_0901, encoding endo-1,4-beta-glucanases
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture genes Tfu_1627, Tfu_1074, Tfu_2176, and Tfu_0901, encoding endo-1,4-beta-glucanases, expression analysis during stationary and exponential growth, overview Thermobifida fusca
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
cells in flasks Thermobifida fusca
0.56
-
cells in bioreactor Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O medium-viscosity substrate Thermobifida fusca ?
-
?

Synonyms

Synonyms Comment Organism
beta-1,4-endoglucanase
-
Thermobifida fusca
Cel5A
-
Thermobifida fusca
Cel6A
-
Thermobifida fusca
Cel9A
-
Thermobifida fusca
Cel9B
-
Thermobifida fusca
endo-1,4-beta-glucanase
-
Thermobifida fusca
endoglucanase
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Thermobifida fusca

General Information

General Information Comment Organism
metabolism the mechanism for enzymatic hydrolysis of cellulose involves synergistic actions by endoglucanase, EC 3.2.1.4, exoglucanase or cellobiohydrolase, EC 3.2.1.91, and beta-glucosidase, EC 3.2.1.21 Thermobifida fusca