Any feedback?
Literature summary for 3.2.1.4 extracted from
- Gene cloning and expression of a new acidic family 7 endo-beta-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1 (2010), Appl. Microbiol. Biotechnol., 85, 1015-1023.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bgl7A, overexpression in Pichia pastoris strain GS115 | Bispora sp. MEY-1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Bispora sp. MEY-1 |  |
| Co2+ | - |
Bispora sp. MEY-1 | |
| Cr3+ | - |
Bispora sp. MEY-1 | |
| Cu2+ | - |
Bispora sp. MEY-1 | |
| EDTA | - |
Bispora sp. MEY-1 | |
| Fe3+ | - |
Bispora sp. MEY-1 | |
| Hg2+ | - |
Bispora sp. MEY-1 | |
| Mg2+ | - |
Bispora sp. MEY-1 | |
| Mn2+ | - |
Bispora sp. MEY-1 | |
| additional information | BglA7 is highly resistant to both pepsin and trypsin | Bispora sp. MEY-1 | |
| Ni2+ | - |
Bispora sp. MEY-1 | |
| Pb2+ | - |
Bispora sp. MEY-1 | |
| SDS | - |
Bispora sp. MEY-1 | |
| Zn2+ | - |
Bispora sp. MEY-1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzymatic activity is enhanced or not affected by the presence of Na+, K+, Li+, Ca2+, or 2-mercaptoethanol | Bispora sp. MEY-1 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE | Bispora sp. MEY-1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bispora sp. MEY-1 | Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bispora sp. MEY-1 | D2DRB6 | acidophilic fungus, CGMCC 2500, gene bgl7A | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bispora sp. MEY-1 |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Bgl7A 1.3fold from Pichia pastoris strain GS115 by ion exchange chromatography | Bispora sp. MEY-1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2740 | - |
substrate lichenan | Bispora sp. MEY-1 |
| 4040 | - |
substrate Hordeum vulgare beta-glucan | Bispora sp. MEY-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | from Hordeum vulgare | Bispora sp. MEY-1 | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
Bispora sp. MEY-1 | ? | - |
? | |
| lichenan + H2O | - |
Bispora sp. MEY-1 | ? | - |
? | |
| additional information | Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan | Bispora sp. MEY-1 | ? | - |
? | |
| additional information | poor activity with laminarin and oat spelt xylan, no activity with Avicel | Bispora sp. MEY-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE | Bispora sp. MEY-1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bgl7A | - |
Bispora sp. MEY-1 |
| endo-beta-1,3-1,4-glucanase | - |
Bispora sp. MEY-1 |
| More | the enzyme belongs to the acidic glycoside hydrolase family 7, GH7 | Bispora sp. MEY-1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bispora sp. MEY-1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | activity rpofile, overview | Bispora sp. MEY-1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant enzyme, 60 min, stable | Bispora sp. MEY-1 |
| 70 | - |
purified enzyme, 48% activity remaining after 5 min, 30% after 60 min | Bispora sp. MEY-1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant Bgl7A has three activity peaks at pH 1.5, pH 3.5, and pH 5.0, the last is the highest | Bispora sp. MEY-1 |
| 5 | - |
- |
Bispora sp. MEY-1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 6 | activity rpofile, overview | Bispora sp. MEY-1 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 8 | purified recombinant enzyme, stable, over 85% within this range | Bispora sp. MEY-1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
