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Literature summary for 3.2.1.4 extracted from

  • Robledo, M.; Jimenez-Zurdo, J.I.; Velazquez, E.; Trujillo, M.E.; Zurdo-Pineiro, J.L.; Ramirez-Bahena, M.H.; Ramos, B.; Diaz-Minguez, J.M.; Dazzo, F.; Martinez-Molina, E.; Mateos, P.F.
    Rhizobium cellulase CelC2 is essential for primary symbiotic infection of legume host roots (2008), Proc. Natl. Acad. Sci. USA, 105, 7064-7069.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Protein Variants

Protein Variants Comment Organism
additional information symbiotic phenotype of an ANU843 celC2 knockout mutant derivative strain ANU843DELTAcelC2 Rhizobium leguminosarum bv. trifolii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km of 84.4 mg/ml for carboxymethyl cellulose as a substrate Rhizobium leguminosarum bv. trifolii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33200
-
-
 Rhizobium leguminosarum bv. trifolii

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum bv. trifolii Q83XK5
-
-
Rhizobium leguminosarum bv. trifolii ANU843 Q83XK5
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Rhizobium leguminosarum bv. trifolii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
152
-
-
 Rhizobium leguminosarum bv. trifolii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Rhizobium leguminosarum bv. trifolii ?
-
 ?
carboxymethyl cellulose + H2O
-
 Rhizobium leguminosarum bv. trifolii ANU843 ?
-
 ?
additional information enzyme does not release reducing sugars from Avicel microcrystalline cellulose, cellobiose, polygalacturonate, locust bean gum, xylan, Rhizobium leguminosarum bv. trifolii acidic heteropolysaccharide EPS types I, II, or III, or Sinorhizobium meliloti succinoglycan exopolysaccharide. Enzyme has a high substrate specificity for noncrystalline cellulose Rhizobium leguminosarum bv. trifolii ?
-
 ?
additional information enzyme does not release reducing sugars from Avicel microcrystalline cellulose, cellobiose, polygalacturonate, locust bean gum, xylan, Rhizobium leguminosarum bv. trifolii acidic heteropolysaccharide EPS types I, II, or III, or Sinorhizobium meliloti succinoglycan exopolysaccharide. Enzyme has a high substrate specificity for noncrystalline cellulose Rhizobium leguminosarum bv. trifolii ANU843 ?
-
 ?

Synonyms

Synonyms Comment Organism
1,4-beta-D-endoglucanase
-
 Rhizobium leguminosarum bv. trifolii
CelC2 cellulase
-
 Rhizobium leguminosarum bv. trifolii
cell-bound bacterial cellulase
-
 Rhizobium leguminosarum bv. trifolii
cellulase CelC2 is essential for primary symbiotic infection of legume host roots Rhizobium leguminosarum bv. trifolii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhizobium leguminosarum bv. trifolii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rhizobium leguminosarum bv. trifolii