Literature summary for 3.2.1.4 extracted from

Hartati, S.; Sudarmonowati, E.; Park, Y.W.; Kaku, T.; Kaida, R.; Baba, K.; Hayashi, T.
Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon (2008), Plant Physiol., 147, 552-561.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in a tropical Leguminosae tree, sengon (Paraserianthes falcataria), by the Agrobacterium tumefaciens method, increases the length and width of stems with larger leaves, which shows a moderately higher density of green color than leaves of the wild type. The pairs of leaves on the transgenic plants close more slowly during sunset than those on the wild-type plants. When main veins from each genotype are excised and placed on a paper towel, however, the leaves of the transgenic plants close more rapidly than those of the wildtype plant. Based on carbohydrate analyses of cell walls, the leaves of the transgenic plants contain less wall-bound xyloglucan than those of the wild-type plants. In situ xyloglucan endotransglucosylase activity shows that the incorporation of whole xyloglucan, potentially for wall tightening, occurrs in the parenchyma cells (motor cells) of the petiolule pulvinus attached to the main vein, although the transgenic plant incorporates less whole xyloglucan than the wild type plant. These observations support the hypothesis that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase, which loosens xyloglucan intercalation, resulting in an irreversible wall modification. This process could be the reason why the overexpression of poplar cellulase both promotes plant growth and disturbs the biological clock of the plant by altering the closing movements of the leaves of the plant	Populus alba

Cloned (Comment)

Organism

overexpressed in a tropical Leguminosae tree, sengon (Paraserianthes falcataria), by the Agrobacterium tumefaciens method, increases the length and width of stems with larger leaves, which shows a moderately higher density of green color than leaves of the wild type. The pairs of leaves on the transgenic plants close more slowly during sunset than those on the wild-type plants. When main veins from each genotype are excised and placed on a paper towel, however, the leaves of the transgenic plants close more rapidly than those of the wildtype plant. Based on carbohydrate analyses of cell walls, the leaves of the transgenic plants contain less wall-bound xyloglucan than those of the wild-type plants. In situ xyloglucan endotransglucosylase activity shows that the incorporation of whole xyloglucan, potentially for wall tightening, occurrs in the parenchyma cells (motor cells) of the petiolule pulvinus attached to the main vein, although the transgenic plant incorporates less whole xyloglucan than the wild type plant. These observations support the hypothesis that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase, which loosens xyloglucan intercalation, resulting in an irreversible wall modification. This process could be the reason why the overexpression of poplar cellulase both promotes plant growth and disturbs the biological clock of the plant by altering the closing movements of the leaves of the plant

Populus alba

Protein Variants

Protein Variants	Comment	Organism
additional information	PopCel1 mRNA is accumulated in seven transgenic lines of Paraserianthes falcataria	Populus alba

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	Western blotting	Populus alba

Organism

Organism	UniProt	Comment	Textmining
Populus alba	Q40763	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellulose + H2O	-	Populus alba	?	-	?
xyloglucan + H2O	-	Populus alba	?	-	?

Synonyms

Synonyms	Comment	Organism
Cellulase	-	Populus alba
PaPopCel1	-	Populus alba