Application | Comment | Organism |
---|---|---|
agriculture | in the treatment of agricultural waste at high temperature and low pH. Utilization in the biofuel industry after thermal pre-treatment in an acidic environment (e.g., steam explosion) of corncob, sugarcane bagasse and several types of agricultural waste to hydrolyze them down to fermentable sugars | Saccharolobus solfataricus |
biofuel production | the enzyme is a candidate for the utilization of agro-industrial waste for fuel production | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Saccharolobus solfataricus | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37300 | - |
x * 37300, calculated from sequence | Saccharolobus solfataricus |
40000 | - |
main active silver-stained band at 57.0 kDa and a light activity at 40.0 kDa (also active towards xylan and carboxymethylcellulose, represents the almost totally deglycosylated SSO1354 protein), SDS-PAGE. Periodic acid Schiff (PAS) staining: only the highest zymogram active band (57.0 kDa) is revealed | Saccharolobus solfataricus |
57000 | - |
main active silver-stained band at 57.0 kDa and a light activity at 40.0 kDa (also active towards xylan and carboxymethylcellulose, represents the almost totally deglycosylated SSO1354 protein), SDS-PAGE. Periodic acid Schiff (PAS) staining: only the highest zymogram active band (57.0 kDa) is revealed | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97YG7 | - |
- |
Saccharolobus solfataricus | Q97YG7 | strains P2, MT4, and Oalpha | - |
Saccharolobus solfataricus Oalpha | Q97YG7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharolobus solfataricus |
glycoprotein | enzyme is highly glycosylated | Saccharolobus solfataricus |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
partially purified, ConA-Sepharose 4B affinity chromatography | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
debranched arabinan, relative activity 51% | Saccharolobus solfataricus |
2.9 | - |
arabinan, relative activity 53% | Saccharolobus solfataricus |
4 | - |
birchwood xylan, relative activity 73% | Saccharolobus solfataricus |
4.2 | - |
oat spelt xylan, relative activity 76% | Saccharolobus solfataricus |
4.6 | - |
beechwood xylan, relative activity 84% | Saccharolobus solfataricus |
5.5 | - |
carboxymethylcellulose, relative activity 100% | Saccharolobus solfataricus |
28.1 | - |
cellulase activity is estimated by the AZO-carboxymethylcellulose standard assay, last purification step | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinan + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
arabinan + H2O | hydrolysed at 53% compared to the hydrolysis of carboxymethylcellulose | Saccharolobus solfataricus | ? | - |
? | |
beechwood xylan + H2O | hydrolysed at 84% compared to the hydrolysis of carboxymethylcellulose | Saccharolobus solfataricus | ? | - |
? | |
birchwood xylan + H2O | hydrolysed at 73% compared to the hydrolysis of carboxymethylcellulose | Saccharolobus solfataricus | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Saccharolobus solfataricus | cellobiose + cello-oligomer | - |
? | |
carboxymethylcellulose + H2O | endo-beta-1,4-glucanase activity. The enzyme shows cellulase and xylanase activity | Saccharolobus solfataricus | cellobiose + cellooligomers | - |
? | |
cellulose + H2O | at 90°C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases | Saccharolobus solfataricus | D-glucose + ? | - |
? | |
oat spelt xylan + H2O | hydrolysed at 76% compared to the hydrolysis of carboxymethylcellulose | Saccharolobus solfataricus | ? | - |
? | |
xylan + H2O | at 90°C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases | Saccharolobus solfataricus | D-xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Saccharolobus solfataricus |
? | x * 37300, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
Cellulase | - |
Saccharolobus solfataricus |
SSO1354 | - |
Saccharolobus solfataricus |
SSO1354 protein | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 110 | enzyme exhibits maximal activity at 95°C and retains 49% activity at 110°C | Saccharolobus solfataricus |
80 | 110 | 80°C: about 40% of maximal activity, 110°C: about 50% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 53 min | Saccharolobus solfataricus |
110 | - |
maximal activity at 95°C and retains 49% activity at 110°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | enzyme is highly active at acidic pH, still exhibiting 48% of the maximal cellulase activities at pH 5.0 | Saccharolobus solfataricus |
3 | 5 | pH 3.0: about 70% of maximal activity, pH 5.0: about 50% of maximal activity | Saccharolobus solfataricus |