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Literature summary for 3.2.1.4 extracted from

  • Maurelli, L.; Giovane, A.; Esposito, A.; Moracci, M.; Fiume, I.; Rossi, M.; Morana, A.
    Evidence that the xylanase activity from Sulfolobus solfataricus Oalpha is encoded by the endoglucanase precursor gene (sso1354) and characterization of the associated cellulase activity (2008), Extremophiles, 12, 689-700.
    View publication on PubMed
Search for more literature for 3.2.1.4

Application

Application Comment Organism
agriculture in the treatment of agricultural waste at high temperature and low pH. Utilization in the biofuel industry after thermal pre-treatment in an acidic environment (e.g., steam explosion) of corncob, sugarcane bagasse and several types of agricultural waste to hydrolyze them down to fermentable sugars Saccharolobus solfataricus
biofuel production the enzyme is a candidate for the utilization of agro-industrial waste for fuel production Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Saccharolobus solfataricus 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37300
-
 x * 37300, calculated from sequence Saccharolobus solfataricus
40000
-
 main active silver-stained band at 57.0 kDa and a light activity at 40.0 kDa (also active towards xylan and carboxymethylcellulose, represents the almost totally deglycosylated SSO1354 protein), SDS-PAGE. Periodic acid Schiff (PAS) staining: only the highest zymogram active band (57.0 kDa) is revealed Saccharolobus solfataricus
57000
-
 main active silver-stained band at 57.0 kDa and a light activity at 40.0 kDa (also active towards xylan and carboxymethylcellulose, represents the almost totally deglycosylated SSO1354 protein), SDS-PAGE. Periodic acid Schiff (PAS) staining: only the highest zymogram active band (57.0 kDa) is revealed Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97YG7
-
-
Saccharolobus solfataricus Q97YG7 strains P2, MT4, and Oalpha
-
Saccharolobus solfataricus Oalpha Q97YG7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Saccharolobus solfataricus
glycoprotein enzyme is highly glycosylated Saccharolobus solfataricus

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus
partially purified, ConA-Sepharose 4B affinity chromatography Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 debranched arabinan, relative activity 51% Saccharolobus solfataricus
2.9
-
 arabinan, relative activity 53% Saccharolobus solfataricus
4
-
 birchwood xylan, relative activity 73% Saccharolobus solfataricus
4.2
-
 oat spelt xylan, relative activity 76% Saccharolobus solfataricus
4.6
-
 beechwood xylan, relative activity 84% Saccharolobus solfataricus
5.5
-
 carboxymethylcellulose, relative activity 100% Saccharolobus solfataricus
28.1
-
 cellulase activity is estimated by the AZO-carboxymethylcellulose standard assay, last purification step Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinan + H2O
-
 Saccharolobus solfataricus ?
-
 ?
arabinan + H2O hydrolysed at 53% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ?
-
 ?
beechwood xylan + H2O hydrolysed at 84% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ?
-
 ?
birchwood xylan + H2O hydrolysed at 73% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ?
-
 ?
carboxymethylcellulose + H2O
-
 Saccharolobus solfataricus cellobiose + cello-oligomer
-
 ?
carboxymethylcellulose + H2O endo-beta-1,4-glucanase activity. The enzyme shows cellulase and xylanase activity Saccharolobus solfataricus cellobiose + cellooligomers
-
 ?
cellulose + H2O at 90°C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases Saccharolobus solfataricus D-glucose + ?
-
 ?
oat spelt xylan + H2O hydrolysed at 76% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ?
-
 ?
xylan + H2O at 90°C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases Saccharolobus solfataricus D-xylose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Saccharolobus solfataricus
? x * 37300, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
Cellulase
-
 Saccharolobus solfataricus
SSO1354
-
 Saccharolobus solfataricus
SSO1354 protein
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 110 enzyme exhibits maximal activity at 95°C and retains 49% activity at 110°C Saccharolobus solfataricus
80 110 80°C: about 40% of maximal activity, 110°C: about 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 half-life: 53 min Saccharolobus solfataricus
110
-
 maximal activity at 95°C and retains 49% activity at 110°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8 enzyme is highly active at acidic pH, still exhibiting 48% of the maximal cellulase activities at pH 5.0 Saccharolobus solfataricus
3 5 pH 3.0: about 70% of maximal activity, pH 5.0: about 50% of maximal activity Saccharolobus solfataricus