Cloned (Comment) | Organism |
---|---|
expressed in Streptomyces lividans TKM31 and Escherichia coli BL21 | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
D261A/R378K | Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center. Mutant has weak chitinase activity, but no soluble products are detected | Thermobifida fusca |
G234S | Cel6B mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center | Thermobifida fusca |
W329C | Cel6B mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center | Thermobifida fusca |
W332A | Cel6B mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center | Thermobifida fusca |
Y206F | Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center | Thermobifida fusca |
Y206S | Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column, followed by a Q-Sepharose column, assessed by SDS-PAGE | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-cellotrioside + H2O | - |
Thermobifida fusca | ? | - |
? | |
alpha-chitin + H2O | due to the binding, it seems chitin would be an inhibitor of Cel6B, Cel9A and Cel48A, but not of Cel6A and Cel5A | Thermobifida fusca | ? | - |
? | |
cellobiose + H2O | - |
Thermobifida fusca | ? | - |
? | |
cellotetraose + H2O | - |
Thermobifida fusca | ? | - |
? | |
cellotriose + H2O | - |
Thermobifida fusca | ? | - |
? | |
cellulose + H2O | the catalytic domains of Cel6A, Cel6B, Cel48A, Cel5A, and Cel9A only show very weak binding to bacterial cellulose | Thermobifida fusca | ? | - |
? | |
chitotetraose + H2O | - |
Thermobifida fusca | ? | - |
? | |
chitotriose + H2O | - |
Thermobifida fusca | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cellulase | - |
Thermobifida fusca |
endocellulase | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Thermobifida fusca |