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Literature summary for 3.2.1.4 extracted from

  • Li, Y.; Wilson, D.B.
    Chitin binding by Thermobifida fusca cellulase catalytic domains (2008), Biotechnol. Bioeng., 100, 644-652.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans TKM31 and Escherichia coli BL21 Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
D261A/R378K Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center. Mutant has weak chitinase activity, but no soluble products are detected Thermobifida fusca
G234S Cel6B mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center Thermobifida fusca
W329C Cel6B mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center Thermobifida fusca
W332A Cel6B mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center Thermobifida fusca
Y206F Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center Thermobifida fusca
Y206S Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column, followed by a Q-Sepharose column, assessed by SDS-PAGE Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-cellotrioside + H2O
-
Thermobifida fusca ?
-
?
alpha-chitin + H2O due to the binding, it seems chitin would be an inhibitor of Cel6B, Cel9A and Cel48A, but not of Cel6A and Cel5A Thermobifida fusca ?
-
?
cellobiose + H2O
-
Thermobifida fusca ?
-
?
cellotetraose + H2O
-
Thermobifida fusca ?
-
?
cellotriose + H2O
-
Thermobifida fusca ?
-
?
cellulose + H2O the catalytic domains of Cel6A, Cel6B, Cel48A, Cel5A, and Cel9A only show very weak binding to bacterial cellulose Thermobifida fusca ?
-
?
chitotetraose + H2O
-
Thermobifida fusca ?
-
?
chitotriose + H2O
-
Thermobifida fusca ?
-
?

Synonyms

Synonyms Comment Organism
Cellulase
-
Thermobifida fusca
endocellulase
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Thermobifida fusca