Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminus truncation mutant Egl330, truncation of the cellulose binding domain, a great improvement in thermal stability is observed in Egl330 | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carboxymethylcellulose | binding of the cellulose-binding domain to avicel is inhibited by carboxymethylcellulose, but not by barley beta-glucan and glucose at concentration of 0.1% and 0.5% | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | Km of Egl330 and Egl499 are 1.73 x 10 mg/ml and 1.14 x 10 mg/ml, respectively. Vmax of Egl330 is 1.78fold higher than that of Egl499, increases from 284 U/mg to 786 U/mg. The catalytic efficiency (kcat/Km) of Egl330 is slightly higher than that of Egl499 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
C-terminus truncation mutant Egl330, calculated | Bacillus subtilis |
36000 | - |
C-terminus truncation mutant Egl330, calculated | Bacillus subtilis |
50000 | - |
Egl499 wild-type, SDS-PAGE | Bacillus subtilis |
55000 | - |
Egl499 wild-type, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
JA18 | - |
Bacillus subtilis JA18 | - |
JA18 | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography on Ni-NTA column | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | soluble substrate | Bacillus subtilis | ? | - |
? | |
barley beta-glucan + H2O | soluble substrate | Bacillus subtilis JA18 | ? | - |
? | |
carboxymethylcellulose + H2O | soluble substrate | Bacillus subtilis | ? | - |
? | |
carboxymethylcellulose + H2O | soluble substrate | Bacillus subtilis JA18 | ? | - |
? | |
cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
cellulose + H2O | - |
Bacillus subtilis JA18 | ? | - |
? | |
additional information | wild-type and mutant can not hydrolyze Avicel, laminarin and chitosan | Bacillus subtilis | ? | - |
? | |
additional information | wild-type and mutant can not hydrolyze Avicel, laminarin and chitosan | Bacillus subtilis JA18 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Egl499 | - |
Bacillus subtilis |
endo-beta-1,4-glucanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | optimal enzymatic reaction temperature tested | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 80 | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis shows both the turnover rate (kcat) and the catalytic efficiency (kcat/Km) of Egl330 increased for the substrate carboxymethylcellulose compared to Egl499 | Bacillus subtilis | |
246 | - |
carboxymethylcellulose | wild-type | Bacillus subtilis | |
437 | - |
carboxymethylcellulose | the turnover rate (kcat) of Egl330 increased by 78% compared to wild-type | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
assay at | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | - |
Bacillus subtilis |