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Literature summary for 3.2.1.4 extracted from

  • Qin, Y.; Wei, X.; Liu, X.; Wang, T.; Qu, Y.
    Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability (2008), Protein Expr. Purif., 58, 162-167.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae. The recombinant Cel5A is heterogeneous, constituted of two components, C1 and C2 Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein different extents of asparagine-linked glycosylation. With increased glycosylation extent of the enzyme components: C2, C1 and native Cel5A, the pH range of activity becomes wider, and thermal stability becomes higher Trichoderma reesei

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
213.1
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Trichoderma reesei ?
-
 ?
ball-milled cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Trichoderma reesei ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel5A
-
 Trichoderma reesei
endoglucanase II
-
 Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6 5 recombinant Cel5A (C1 and C2) from Saccharomyces cerevisiae Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
4 6.4 pH 4.0: about 80% of maximal activity, pH 6.4: about 60% of maximal activity, recombinant Cel5A Trichoderma reesei