Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Gymnascella citrina | |
Mn2+ | above 5 mM | Gymnascella citrina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value for carboxymethylcellulase is 6.25 mg/ml at pH 5.0, 50°C | Gymnascella citrina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the removal of metals results into complete loss of enzymatic activity and is completely recovered in the presence of 1 mM Mn2+. 12fold activation at 1.5 mM Mn2+ | Gymnascella citrina | |
additional information | no inhibition up to 7 mM of Ca2+, Zn2+ and K+ is observed | Gymnascella citrina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
1 * 42000, SDS-PAGE | Gymnascella citrina |
43000 | - |
gel filtratiion | Gymnascella citrina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gymnascella citrina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gymnascella citrina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
115 | - |
- |
Gymnascella citrina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Gymnascella citrina | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Gymnascella citrina | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Gymnascella citrina |
Synonyms | Comment | Organism |
---|---|---|
CMCase-I | - |
Gymnascella citrina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Gymnascella citrina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
318 | - |
carboxymethyl cellulose | in presence of 1.5 mM Mn2+ | Gymnascella citrina |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | stable | Gymnascella citrina |