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Literature summary for 3.2.1.4 extracted from

  • Voget, S.; Steele, H.L.; Streit, W.R.
    Characterization of a metagenome-derived halotolerant cellulase (2006), J. Biotechnol., 126, 26-36.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Dimethylsulfoxide above 5-15% v/v unidentified microorganism

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli unidentified microorganism

General Stability

General Stability Organism
high salt stability, the enzyme retained 86-87% activity after incubation with 3 M NaCl, 3 M RbCl or 4 M KCl for 20 h unidentified microorganism

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 10% v/v, 89% loss of activity unidentified microorganism
Ba2+ 1 mM, 79%inhibition unidentified microorganism
Ca2+ 1 mM, 74%inhibition unidentified microorganism
Cu2+ 1 mM, 12% loss of activity unidentified microorganism
Dimethylsulfoxide 30% v/v, 50% inhibition unidentified microorganism
EDTA 50 mM, 14% inhibition unidentified microorganism
ethanol 30% v/v, 92% loss of activity unidentified microorganism
Fe3+ 1 mM, 73% inhibition unidentified microorganism
methanol 30% v/v, 62% loss of activity unidentified microorganism
Mn2+ 5 mM, 20% inhibition unidentified microorganism
SDS 1% v/v, almost complete loss of activity unidentified microorganism
Zn2+ 1 mM, 78%inhibition unidentified microorganism

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 1.3fold activation unidentified microorganism

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE unidentified microorganism
42100
-
x * 42100, calculated from sequence unidentified microorganism

Organism

Organism UniProt Comment Textmining
unidentified microorganism
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
unidentified microorganism

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellopentaose + H2O
-
unidentified microorganism cellobiose + glucose
-
?
additional information no activity against crystalline forms of cellulose such as filter-paper or avicel and the beta-1,3-linked glucan laminarin. No activity with xylan unidentified microorganism ?
-
?
soluble cellulose + H2O endo mode of action. At the beginning only high molecular mass products are released suggesting an endowise action of the enzyme. The major product has a degree of polymerization of three, minor quantities of smaller and larger oligosaccharides are also produced. Not active against insoluble cellulose unidentified microorganism ?
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE unidentified microorganism
? x * 42100, calculated from sequence unidentified microorganism

Synonyms

Synonyms Comment Organism
Cel5A
-
unidentified microorganism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
unidentified microorganism

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: 76% of maximal activity, 80°C: 89% of maximal activity unidentified microorganism

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to 11 days unidentified microorganism
55
-
10 min, 80% loss of activity unidentified microorganism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
unidentified microorganism

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.5: 60% of maximal activity, pH 9: 68% of maximal activity unidentified microorganism