Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dimethylsulfoxide | above 5-15% v/v | unidentified microorganism |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | unidentified microorganism |
General Stability | Organism |
---|---|
high salt stability, the enzyme retained 86-87% activity after incubation with 3 M NaCl, 3 M RbCl or 4 M KCl for 20 h | unidentified microorganism |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | 10% v/v, 89% loss of activity | unidentified microorganism | |
Ba2+ | 1 mM, 79%inhibition | unidentified microorganism | |
Ca2+ | 1 mM, 74%inhibition | unidentified microorganism | |
Cu2+ | 1 mM, 12% loss of activity | unidentified microorganism | |
Dimethylsulfoxide | 30% v/v, 50% inhibition | unidentified microorganism | |
EDTA | 50 mM, 14% inhibition | unidentified microorganism | |
ethanol | 30% v/v, 92% loss of activity | unidentified microorganism | |
Fe3+ | 1 mM, 73% inhibition | unidentified microorganism | |
methanol | 30% v/v, 62% loss of activity | unidentified microorganism | |
Mn2+ | 5 mM, 20% inhibition | unidentified microorganism | |
SDS | 1% v/v, almost complete loss of activity | unidentified microorganism | |
Zn2+ | 1 mM, 78%inhibition | unidentified microorganism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 1.3fold activation | unidentified microorganism |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | unidentified microorganism |
42100 | - |
x * 42100, calculated from sequence | unidentified microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified microorganism | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
unidentified microorganism |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellopentaose + H2O | - |
unidentified microorganism | cellobiose + glucose | - |
? | |
additional information | no activity against crystalline forms of cellulose such as filter-paper or avicel and the beta-1,3-linked glucan laminarin. No activity with xylan | unidentified microorganism | ? | - |
? | |
soluble cellulose + H2O | endo mode of action. At the beginning only high molecular mass products are released suggesting an endowise action of the enzyme. The major product has a degree of polymerization of three, minor quantities of smaller and larger oligosaccharides are also produced. Not active against insoluble cellulose | unidentified microorganism | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | unidentified microorganism |
? | x * 42100, calculated from sequence | unidentified microorganism |
Synonyms | Comment | Organism |
---|---|---|
Cel5A | - |
unidentified microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
unidentified microorganism |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: 76% of maximal activity, 80°C: 89% of maximal activity | unidentified microorganism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to 11 days | unidentified microorganism |
55 | - |
10 min, 80% loss of activity | unidentified microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
unidentified microorganism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: 60% of maximal activity, pH 9: 68% of maximal activity | unidentified microorganism |