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Literature summary for 3.2.1.4 extracted from

  • Rajoka, M.I.; Ashraf, Y.; Khalid, A.M.
    Kinetic of improved production and carboxymethyl cellulose hydrolysis by an endo-glucanase from a derepressed mutant of Cellulomonas biazotea (2004), Biotechnol. Lett., 26, 1329-1333.
    View publication on PubMed
Search for more literature for 3.2.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-value for carboxymethyl cellulose: 3.5% w/v (enzyme from strain NIAB442), 2.5% w/v (enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr) Cellulomonas biazotea

Organism

Organism UniProt Comment Textmining
Cellulomonas biazotea
-
 strain NIAB 442 and its deoxyglucose- and streptomycin-resistant mutant 51 Smr
-
Cellulomonas biazotea NIAB 442
-
 strain NIAB 442 and its deoxyglucose- and streptomycin-resistant mutant 51 Smr
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cellulomonas biazotea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Cellulomonas biazotea D-glucose + ?
-
 ?
carboxymethyl cellulose + H2O
-
 Cellulomonas biazotea NIAB 442 D-glucose + ?
-
 ?
carboxymethylcellulose + H2O
-
 Cellulomonas biazotea ?
-
 ?
carboxymethylcellulose + H2O
-
 Cellulomonas biazotea NIAB 442 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
217
-
 carboxymethyl cellulose enzyme from strain NIAB442 Cellulomonas biazotea
278
-
 carboxymethyl cellulose enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr Cellulomonas biazotea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 enzyme from strain NIAB442 Cellulomonas biazotea
7
-
 enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr Cellulomonas biazotea