Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Yoda, K.; Toyoda, A.; Mukoyama, Y.; Nakamura, Y.; Minato, H.
    Cloning, sequencing, and expression of a Eubacterium cellulosolvens 5 gene encoding an endoglucanase (Cel5A) with novel carbohydrate-binding modules, and properties of Cel5A (2005), Appl. Environ. Microbiol., 71, 5787-5793.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli [Eubacterium] cellulosolvens

Protein Variants

Protein Variants Comment Organism
additional information the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function. Removal of the carbohydrate-binding modules from rCel5A reduces the catalytic activities with various polysaccharides remarkably [Eubacterium] cellulosolvens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
127047
-
x * 127047, the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function, MW calculated from sequence [Eubacterium] cellulosolvens

Organism

Organism UniProt Comment Textmining
[Eubacterium] cellulosolvens Q3LHN3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Eubacterium] cellulosolvens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid-swollen cellulose + H2O
-
[Eubacterium] cellulosolvens ?
-
?
carboxymethyl cellulose + H2O
-
[Eubacterium] cellulosolvens ?
-
?
lichenan + H2O
-
[Eubacterium] cellulosolvens ?
-
?
additional information the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function. Removal of the carbohydrate-binding modules from rCel5A reduces the catalytic activities with various polysaccharides remarkably [Eubacterium] cellulosolvens ?
-
?
oat spelt xylan + H2O
-
[Eubacterium] cellulosolvens ?
-
?

Subunits

Subunits Comment Organism
? x * 127047, the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function, MW calculated from sequence [Eubacterium] cellulosolvens

Synonyms

Synonyms Comment Organism
Cel5A
-
[Eubacterium] cellulosolvens
endoglucanase Cel5A
-
[Eubacterium] cellulosolvens