Cloned (Comment) | Organism |
---|---|
N-terminally truncated mutant DELTA1-90 is expressed as a soluble protein in Pichia pastoris | Brassica napus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-90 | expressed as a soluble protein in Pichia pastoris, the wild-type enzyme is anchored to membrane | Brassica napus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, complete inhibition of mutant enzyme DELTA1-90 | Brassica napus | |
Mg2+ | above 10 mM | Brassica napus | |
Zn2+ | above 5 mM | Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | wild-type enzyme is anchored to membrane | Brassica napus | 16020 | - |
soluble | the N-terminally truncated mutant DELTA1-90 is expressed as a soluble protein in Pichia pastoris | Brassica napus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activity of mutant enzyme DELTA1-90 is enhances in a concentration-dependent manner up to about 40 mM | Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
x * 130000, mutant enzyme DELTA1-90, SDS-PAGE | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | Q9STD9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | MW detected by SDS-PAGE is 130000 Da, after removal of N-glycans the MW is 60000 Da | Brassica napus |
Purification (Comment) | Organism |
---|---|
mutant enzyme DELTA1-90 | Brassica napus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Brassica napus | ? | - |
? | |
carboxymethylcellulose + H2O | activity with carbomethylcellulose with approxomately 4 carboxymethyl groups per 10 anhydro-glucose units is about 3.5 times better than that with carbomethylcellulose with approximately 8 carboxymethyl groups | Brassica napus | ? | - |
? | |
additional information | no activity with tamarind xyloglucan, barley (1-3)(1-4)-beta-D-glucan, wheat arabinoxylan or birchwood xylan | Brassica napus | ? | - |
? | |
phosphoric acid-swollen cellulose + H2O | - |
Brassica napus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, mutant enzyme DELTA1-90, SDS-PAGE | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
mutant enzyme DELTA1-90, hydrolysis of carbomethylcellulose with approximately 4 carboxymethyl groups per 10 anhydro-glucose units | Brassica napus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.4 | pH 5.8: about 55% of maximal activity, pH 6.4: about 55% of maximal activity, mutant enzyme DELTA1-90, hydrolysis of carbomethylcellulose with approxomately 4 carboxymethyl groups per 10 anhydro-glucose units | Brassica napus |