Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Belaich, A.; Parsiegla, G.; Gal, L.; Villard, C.; Haser, R.; Belaich, J.P.
    Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome (2002), J. Bacteriol., 184, 1378-1384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ruminiclostridium cellulolyticum

Protein Variants

Protein Variants Comment Organism
E410Q mutation totally inactivates carboxymethyl cellulase activity of the protein Ruminiclostridium cellulolyticum

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54618
-
x * 54618, the enzyme contains a catalytic domain belonging to family 9 and a dockering domain, it is a component of the cellulosome, calculation from nucleotide sequence Ruminiclostridium cellulolyticum

Organism

Organism UniProt Comment Textmining
Ruminiclostridium cellulolyticum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ruminiclostridium cellulolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O weak activity Ruminiclostridium cellulolyticum ?
-
?
bacterial microcrystalline cellulose + H2O weak activity Ruminiclostridium cellulolyticum cellotetraose 83% cellotetraose + 11% cellotriose + 4% cellobiose ?
carboxymethylcellulose + H2O
-
Ruminiclostridium cellulolyticum ?
-
?
cellopentaose + H2O
-
Ruminiclostridium cellulolyticum cellotetraose + glucose
-
?
additional information no cleavage of cellotetraose, cellotriose and p-nitrophenyl-cellobiose Ruminiclostridium cellulolyticum ?
-
?
phosphoric acid-swollen cellulose + H2O weak Ruminiclostridium cellulolyticum ?
-
?

Subunits

Subunits Comment Organism
? x * 54618, the enzyme contains a catalytic domain belonging to family 9 and a dockering domain, it is a component of the cellulosome, calculation from nucleotide sequence Ruminiclostridium cellulolyticum

Synonyms

Synonyms Comment Organism
Cel9M
-
Ruminiclostridium cellulolyticum