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Literature summary for 3.2.1.4 extracted from
- Purification and characterization of a protease-resistant cellulase from Aspergillus niger (1995), J. Ferment. Bioeng., 79, 125-130.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is very strongly protease resistant, not affected by 2.5 mg/ml savinase, 250 mg/l proteinase K, 250 mg/l pronase E, and 2.5 mg/ml trypsin in 50 mM Tris buffer, pH 7.5, after 3 days. 70% loss of activity after incubation with savinase for 2 weeks | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 2 mM | Aspergillus niger |  |
| Hg2+ | - |
Aspergillus niger | |
| additional information | more than 50% of the original activity is retained after incubation with sodium alkyl sulfonate, sodium alkyl sulfate, polyoxyethylene alkyl sulfonate, alpha-olefin sulfonate | Aspergillus niger | |
| N-bromosuccinimide | - |
Aspergillus niger | |
| Sn2+ | 2 mM | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
IFO31125 | - |
| Aspergillus niger IFO31125 | - |
IFO31125 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 8.9% carbohydrate | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 194 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Aspergillus niger | additional information | - |
? | |
| carboxymethylcellulose + H2O | - |
Aspergillus niger IFO31125 | additional information | - |
? | |
| cellulose + H2O | acid-swollen | Aspergillus niger | ? | - |
? | |
| cellulose + H2O | acid-swollen | Aspergillus niger IFO31125 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
2 h, 30% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | more than 50% of maximal activity at pH 4.0 and at pH 8.0 | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 40°C, stable for at least 24 h | Aspergillus niger |
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Release 2023.1 (January 2023)
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