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Literature summary for 3.2.1.4 extracted from

  • Watanabe, Y.; Moriyama, R.; Matsuda, T.; Shimizu, S.; Ohmiya, K.
    Purification and properties of the endo-1,4-beta-glucanase III from Ruminococcus albus (1992), J. Ferment. Bioeng., 73, 54-57.
No PubMed abstract available
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation Ruminococcus albus
Cysteine hydrochloride slight activation Ruminococcus albus
dithiothreitol slight activation Ruminococcus albus
glutathione slight activation Ruminococcus albus

General Stability

General Stability Organism
2-mercaptoethanol, dithiothreitol, glutathione and cysteine-HCl stabilize Ruminococcus albus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Ruminococcus albus
Fe2+
-
 Ruminococcus albus
Hg2+
-
 Ruminococcus albus
iodoacetamide
-
 Ruminococcus albus
NEM
-
 Ruminococcus albus
PCMB
-
 Ruminococcus albus
Zn2+
-
 Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.72
-
 cellohexaose
-
 Ruminococcus albus
7.24
-
 cellopentaose
-
 Ruminococcus albus
8.85
-
 cellotetraose
-
 Ruminococcus albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 gel filtration Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus
-
 endoglucanase III
-

Purification (Commentary)

Purification (Comment) Organism
endoglucanase III Ruminococcus albus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
 Ruminococcus albus additional information
-
 ?
cellohexaose + H2O
-
 Ruminococcus albus additional information
-
 ?
cellopentaose + H2O
-
 Ruminococcus albus additional information
-
 ?
cellotetraose + H2O
-
 Ruminococcus albus 2 cellobiose
-
 ?
cellotriose + H2O negligibly hydrolyzed Ruminococcus albus D-glucose + cellobiose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 carboxymethylcellulose-degrading activity Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
 carboxymethylcellulose-degrading activity Ruminococcus albus