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Literature summary for 3.2.1.4 extracted from
- Purification and properties of extracellular and cell-bound cellulase components of Pseudomonas fluorescens var. cellulosa (1970), J. Biochem., 67, 19-35.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell bound | cellulase C | Pseudomonas fluorescens | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
var. cellulosa | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycoprotein | Pseudomonas fluorescens |
| glycoprotein | galactose and glucose are the major constituent sugars. Glucosamine is detected in both cellulase A and B. Galactosamine is detected in cellulase C | Pseudomonas fluorescens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cellulase A, B and C | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Pseudomonas fluorescens | additional information | - |
? |  |
| cellodextrin + H2O | cellulase A, B, and C | Pseudomonas fluorescens | ? | - |
? | |
| cellohexaose + H2O | cellulase A, B, and C | Pseudomonas fluorescens | additional information | - |
? | |
| cellopentaose + H2O | cellulase A, B, and C | Pseudomonas fluorescens | additional information | - |
? | |
| cellopentaosyl sorbitol + H2O | cellulase A, B, and C | Pseudomonas fluorescens | ? | - |
? | |
| cellotetraose + H2O | cellulase A, B, and C | Pseudomonas fluorescens | 2 cellobiose | - |
? | |
| cellotetraosyl sorbitol + H2O | cellulase A, B, and C | Pseudomonas fluorescens | ? | - |
? | |
| cellotriose + H2O | cellulase C | Pseudomonas fluorescens | D-glucose + cellobiose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
slight activation of cellulase B after 10 min at 40°C | Pseudomonas fluorescens |
| 60 | - |
10 min, complete inactivation of cellulase A, B and C | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
cellulase C | Pseudomonas fluorescens |
| 8 | - |
cellulase A and B | Pseudomonas fluorescens |
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