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Literature summary for 3.2.1.4 extracted from
- The cellulase of Trichoderma koningii. Purification and properties of some endoglucanase components with special reference to their action on cellulose when acting alone and in synergism with the cellobiohydrolase (1978), Biochem. J., 171, 61-72.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | 0.29 mM, stimulation | Trichoderma koningii |  |
| methylcellulose | 0.1%, 20% stimulation of carboxymethylcellulase activity of enzyme E1 | Trichoderma koningii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | inhibition of endoglucanase E3a, E3b and E4 | Trichoderma koningii | |
| glucosamine | inhibition of CM-cellulase activity of enzyme E1, E3a, E3b and E4 | Trichoderma koningii | |
| glucose | 29 mM, 12% inhibition of endoglucanase E4, no inhibition of endoglucanase E1 and E3a | Trichoderma koningii | |
| methylcellulose | poor inhibitor of carboxymethylcellulase activity of enzyme form E3a, E3b and E4 | Trichoderma koningii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13000 | - |
endoglucanase E1, gel filtration | Trichoderma koningii |
| 31000 | - |
endoglucanase E4, gel filtration | Trichoderma koningii |
| 48000 | - |
enoglucanase E3a, gel filtration | Trichoderma koningii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma koningii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| endoglucanase E1, E3a and E4 | Trichoderma koningii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Trichoderma koningii | additional information | - |
? | |
| cellopentaose + H2O | - |
Trichoderma koningii | additional information | - |
? | |
| cellotetraose + H2O | - |
Trichoderma koningii | 2 cellobiose | - |
? | |
| cellulose + H2O | acid-swollen | Trichoderma koningii | ? | - |
? | |
| additional information | hydrolysis of cotton | Trichoderma koningii | ? | - |
? | |
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Release 2023.1 (January 2023)
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