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Literature summary for 3.2.1.4 extracted from
- Purification and characterization of a cellulase from Dolabella auricularia (1984), J. Biochem., 96, 1381-1390.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Dolabella auricularia |  |
| Hg2+ | - |
Dolabella auricularia | |
| Mn2+ | - |
Dolabella auricularia | |
| N-bromosuccinimide | 0.001 mM, complete inactivation | Dolabella auricularia | |
| SDS | 0.25 mM, complete inactivation | Dolabella auricularia | |
| Zn2+ | - |
Dolabella auricularia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Dolabella auricularia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dolabella auricularia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dolabella auricularia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 277.5 | - |
- |
Dolabella auricularia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Dolabella auricularia | additional information | - |
? | |
| cellohexaose + H2O | - |
Dolabella auricularia | additional information | cellobiose + cellotetraose | ? | |
| cellopentaose + H2O | - |
Dolabella auricularia | additional information | cellotetraose + glucose | ? | |
| cellotetraose + H2O | - |
Dolabella auricularia | 2 cellobiose | - |
? | |
| cellulose + H2O | acid-swollen | Dolabella auricularia | ? | - |
? | |
| additional information | when the oligosaccharides are modified by reduction with sodium borotritide, the second linkage from the reducing end becomes significantly susceptible to the enzyme and is preferentially cleaved | Dolabella auricularia | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Dolabella auricularia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable up to | Dolabella auricularia |
| 55 | - |
10 min, complete inactivation | Dolabella auricularia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Dolabella auricularia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | 0°C, 12 h, stable | Dolabella auricularia |
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