Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli in a correctly processed form | Streptomyces rochei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, catalytic domain, SDS-PAGE | Streptomyces rochei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rochei | - |
A2 | - |
Streptomyces rochei A2 | - |
A2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
64.8 | - |
catalytic domain expressed in Escherichia coli | Streptomyces rochei |
Storage Stability | Organism |
---|---|
4°C, catalytic domain is stable for at least 1 week | Streptomyces rochei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | - |
Streptomyces rochei | ? | - |
? | |
cellulose + H2O | - |
Streptomyces rochei A2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, catalytic domain, SDS-PAGE | Streptomyces rochei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
catalytic domain | Streptomyces rochei |
65 | - |
whole enzyme | Streptomyces rochei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | 37°C: about 50% of maximal activity of the catalytic domain and of the whole enzyme, 80°C: about 30% of maximal activity of the catalytic domain, about 45% of maximal activity of the whole enzyme | Streptomyces rochei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, more than 50% loss of activity of the catalytic domain and of the whole enzyme | Streptomyces rochei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
catalytic domain | Streptomyces rochei |
6 | - |
whole enzyme | Streptomyces rochei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | pH 4.0: about 25% of maximal activity of the whole enzyme about 45% of maximal activity of the catalytic domain, pH 8.0: about 45% of maximal activity of the catalytic domain, about 55% of maximal activity of the whole enzyme | Streptomyces rochei |