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Literature summary for 3.2.1.4 extracted from

  • Aa, K.; Flengsrud, R.; Lindahl, V.; Tronsmo, A.
    Characterization of production and enzyme properties of an endo-beta-1,4-glucanase from Bacillus subtilis CK-2 isolated from compost soil (1994), Antonie van Leeuwenhoek, 66, 319-326.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Bacillus subtilis
mercaptoethanol
-
Bacillus subtilis
Mn2+
-
Bacillus subtilis
SDS
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
2 * 35500, endo-beta-1,4-glucanase E1, E2 and E3, SDS-PAGE Bacillus subtilis
70000
-
gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
CK-2
-
Bacillus subtilis CK-2
-
CK-2
-

Purification (Commentary)

Purification (Comment) Organism
endo-beta-1,4-glucanase E1, E2 and E3 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.1
-
endo-beta-1,4-glucanase E1 Bacillus subtilis
66.4
-
endo-beta-1,4-glucanase E2 and E3 Bacillus subtilis

Storage Stability

Storage Stability Organism
4°C, endo-beta-1,4-glucanase E2 and E2, stable for several weeks Bacillus subtilis

Subunits

Subunits Comment Organism
dimer 2 * 35500, endo-beta-1,4-glucanase E1, E2 and E3, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min, stable up to Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 5.8
-
Bacillus subtilis