Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | AgNO3, endoglucanase E1 and E2 | Thermobifida fusca | |
Hg2+ | endoglucanase E1 and E2; HgCl2 | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme E2 and E3 are glycoproteins | Thermobifida fusca |
no modification | enzyme E1, E4 and E5 are not glycoproteins | Thermobifida fusca |
Purification (Comment) | Organism |
---|---|
enzyme form E1, E2, E3, E4 and E5 | Thermobifida fusca |
Storage Stability | Organism |
---|---|
-70°C, purified enzymes are very stable | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Thermobifida fusca | additional information | - |
? | |
cellotriose + H2O | hydrolyzed by endoglucanase E1, no activity with endoglucanase E2 | Thermobifida fusca | D-glucose + cellobiose | + glucose, endoglucanase E1 | ? | |
cellulose + H2O | amorphous cellulose | Thermobifida fusca | ? | endoglucanase E1 produces mainly cellobiose and a trace of glucose, endoglucanase E2 produces mainly cellotriose and higher oligomers with some cellobiose and a trace of glucose | ? | |
xylan + H2O | - |
Thermobifida fusca | additional information | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
endoglucanase E1 and E2 | Thermobifida fusca |