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Literature summary for 3.2.1.4 extracted from

  • Halliwell, G.; Halliwell, N.
    Cellulolytic enzyme components of the cellulase complex of Clostridium thermocellum (1989), Biochim. Biophys. Acta, 992, 223-229.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
endoglucanase 1b, non-denaturing PAGE Acetivibrio thermocellus
64000
-
endoglucanase 1b, gel filtration Acetivibrio thermocellus
100000
-
endoglucanase 2, gel filtration Acetivibrio thermocellus
110000
-
endoglucanase 2, non-denaturing PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
endoglucanase 1b and 2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Acetivibrio thermocellus additional information endoglucanase 1b: cellotriose, 45%, cellotetraose, 31%, cellobiose, 17%, and cellopentaose, 8%. Endoglucanase 2: cellotriose, 50%, and cellotetraose, 30% ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
endoglucanase 1b Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 50% of maximal activity, pH 8.0: about 45% of maximal activity, endoglucanase 1b Acetivibrio thermocellus