Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | catalytic activity is promoted in the presence of DMSO. DMSO affects the oligomerization state, causing the enzyme dimers to associate into tetramers | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged protein | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
amylopectin | Km-values: 31.1 mg/ml at pH 5.5, 75°C, in absence of DMSO. 88.0 mg/ml at pH 5.5, 75°C, in presence of DMSO. 92.3 mg/ml at pH 6.5, 75°C, in absence of DMSO. 345 mg/ml at pH 5.5, 75°C, in presence of DMSO | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
2 * 83000, the enzyme is mainly dimeric at pH 7.0, SDS-PAGE | Saccharolobus solfataricus |
83000 | - |
4 * 83000, the enzyme is mainly dimeric at pH 5.5-6.5, SDS-PAGE | Saccharolobus solfataricus |
93091 | - |
2 * 93091, the enzyme is mainly dimeric at pH 7.0, calculated from sequence | Saccharolobus solfataricus |
93091 | - |
4 * 93091, the enzyme is mainly dimeric at pH 5.5-6.5, calculated from sequence | Saccharolobus solfataricus |
141000 | - |
dimeric enzyme form, analytical ultracentrfugation | Saccharolobus solfataricus |
355000 | - |
tetrameric enzyme form, analytical ultracentrfugation | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q7LX99 | - |
- |
Saccharolobus solfataricus P2 | Q7LX99 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-O-alpha-D-maltosyl-beta-cyclodextrin + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
6-O-alpha-D-maltosyl-beta-cyclodextrin + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
amylopectin + H2O | - |
Saccharolobus solfataricus | maltose + ? | - |
? | |
amylopectin + H2O | - |
Saccharolobus solfataricus P2 | maltose + ? | - |
? | |
branched cyclodextrin + H2O | the TreX protein has a specificity for hydrolysis of chains consisting of 6 glucose residues or longer | Saccharolobus solfataricus | ? | - |
? | |
branched cyclodextrin + H2O | the TreX protein has a specificity for hydrolysis of chains consisting of 6 glucose residues or longer | Saccharolobus solfataricus P2 | ? | - |
? | |
glycogen + H2O | preferred substrate | Saccharolobus solfataricus | ? | - |
? | |
glycogen + H2O | preferred substrate | Saccharolobus solfataricus P2 | ? | - |
? | |
pullulan + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
pullulan + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 83000, the enzyme is mainly dimeric at pH 7.0, SDS-PAGE | Saccharolobus solfataricus |
dimer | 2 * 93091, the enzyme is mainly dimeric at pH 7.0, calculated from sequence | Saccharolobus solfataricus |
tetramer | 4 * 83000, the enzyme is mainly dimeric at pH 5.5-6.5, SDS-PAGE | Saccharolobus solfataricus |
tetramer | 4 * 93091, the enzyme is mainly dimeric at pH 5.5-6.5, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SSO2094 | locus name | Saccharolobus solfataricus |
TreX | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 80 | 65°C: about 45% of maximal activity, 80°C: about 80% of maximal activity | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
amylopectin | pH 6.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO | Saccharolobus solfataricus | |
0.923 | - |
amylopectin | pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO | Saccharolobus solfataricus | |
7.57 | - |
amylopectin | pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO | Saccharolobus solfataricus | |
17.35 | - |
amylopectin | pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | pH 5.0: about 20% of maximal activity, pH 6.5: about 85% of maximal activity, pH 7.0: about 15% of maximal activity | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme may be involved in glycogen metabolism in debranching and rearranging the side chain of the glycogen by both alpha-1,6-hydrolase and alpha-1,6-transferase activity | Saccharolobus solfataricus |