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Literature summary for 3.2.1.33 extracted from

  • Park, J.; Park, H.; Kang, H.; Hong, J.; Cha, H.; Woo, E.; Kim, J.; Kim, M.; Boos, W.; Lee, S.; Park, K.
    Oligomeric and functional properties of a debranching enzyme (TreX) from the archaeon Sulfolobus solfataricus P2 (2008), Biocatal. Biotransform., 26, 76-85.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
DMSO catalytic activity is promoted in the presence of DMSO. DMSO affects the oligomerization state, causing the enzyme dimers to associate into tetramers Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged protein Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
amylopectin Km-values: 31.1 mg/ml at pH 5.5, 75°C, in absence of DMSO. 88.0 mg/ml at pH 5.5, 75°C, in presence of DMSO. 92.3 mg/ml at pH 6.5, 75°C, in absence of DMSO. 345 mg/ml at pH 5.5, 75°C, in presence of DMSO Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
2 * 83000, the enzyme is mainly dimeric at pH 7.0, SDS-PAGE Saccharolobus solfataricus
83000
-
4 * 83000, the enzyme is mainly dimeric at pH 5.5-6.5, SDS-PAGE Saccharolobus solfataricus
93091
-
2 * 93091, the enzyme is mainly dimeric at pH 7.0, calculated from sequence Saccharolobus solfataricus
93091
-
4 * 93091, the enzyme is mainly dimeric at pH 5.5-6.5, calculated from sequence Saccharolobus solfataricus
141000
-
dimeric enzyme form, analytical ultracentrfugation Saccharolobus solfataricus
355000
-
tetrameric enzyme form, analytical ultracentrfugation Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q7LX99
-
-
Saccharolobus solfataricus P2 Q7LX99
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-alpha-D-maltosyl-beta-cyclodextrin + H2O
-
Saccharolobus solfataricus ?
-
?
6-O-alpha-D-maltosyl-beta-cyclodextrin + H2O
-
Saccharolobus solfataricus P2 ?
-
?
amylopectin + H2O
-
Saccharolobus solfataricus maltose + ?
-
?
amylopectin + H2O
-
Saccharolobus solfataricus P2 maltose + ?
-
?
branched cyclodextrin + H2O the TreX protein has a specificity for hydrolysis of chains consisting of 6 glucose residues or longer Saccharolobus solfataricus ?
-
?
branched cyclodextrin + H2O the TreX protein has a specificity for hydrolysis of chains consisting of 6 glucose residues or longer Saccharolobus solfataricus P2 ?
-
?
glycogen + H2O preferred substrate Saccharolobus solfataricus ?
-
?
glycogen + H2O preferred substrate Saccharolobus solfataricus P2 ?
-
?
pullulan + H2O
-
Saccharolobus solfataricus ?
-
?
pullulan + H2O
-
Saccharolobus solfataricus P2 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 83000, the enzyme is mainly dimeric at pH 7.0, SDS-PAGE Saccharolobus solfataricus
dimer 2 * 93091, the enzyme is mainly dimeric at pH 7.0, calculated from sequence Saccharolobus solfataricus
tetramer 4 * 83000, the enzyme is mainly dimeric at pH 5.5-6.5, SDS-PAGE Saccharolobus solfataricus
tetramer 4 * 93091, the enzyme is mainly dimeric at pH 5.5-6.5, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO2094 locus name Saccharolobus solfataricus
TreX
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 80 65°C: about 45% of maximal activity, 80°C: about 80% of maximal activity Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
amylopectin pH 6.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO Saccharolobus solfataricus
0.923
-
amylopectin pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO Saccharolobus solfataricus
7.57
-
amylopectin pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO Saccharolobus solfataricus
17.35
-
amylopectin pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 pH 5.0: about 20% of maximal activity, pH 6.5: about 85% of maximal activity, pH 7.0: about 15% of maximal activity Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the enzyme may be involved in glycogen metabolism in debranching and rearranging the side chain of the glycogen by both alpha-1,6-hydrolase and alpha-1,6-transferase activity Saccharolobus solfataricus