Cloned (Comment) | Organism |
---|---|
GUS, DNA and amino acid sequence determination, analysis and comparison, overexpression of the His-tagged enzyme in Escherichia coli strain GMS407 | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
x * 71000, recombinant enzyme, SDS-PAGE | Levilactobacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Levilactobacillus brevis RO1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GUS from Escherichia coli strain GMS407 | Levilactobacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1284 | - |
purified recombinant enzyme | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis | 4-nitrophenol + beta-D-glucuronate | - |
? | |
4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis RO1 | 4-nitrophenol + beta-D-glucuronate | - |
? | |
baicalin + H2O | - |
Levilactobacillus brevis | baicalein + beta-D-glucuronate | - |
? | |
baicalin + H2O | - |
Levilactobacillus brevis RO1 | baicalein + beta-D-glucuronate | - |
? | |
wogonin 7-O-beta-D-glucuronide + H2O | - |
Levilactobacillus brevis | wogonin + beta-D-glucuronate | - |
? | |
wogonin 7-O-beta-D-glucuronide + H2O | - |
Levilactobacillus brevis RO1 | wogonin + beta-D-glucuronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, recombinant enzyme, SDS-PAGE | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
GUS | - |
Levilactobacillus brevis |
More | the enzyme belongs to glycosyl hydrolase family 2 with three conserved domains | Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Levilactobacillus brevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
80 min, purified recombinant enzyme, pH 5.0-8.0, completely stable | Levilactobacillus brevis |
50 | - |
purified recombinant enzyme, half-life is over 2 h | Levilactobacillus brevis |
60 | - |
purified recombinant enzyme, half-life is 1 h | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant enzyme | Levilactobacillus brevis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 80 min, purified recombinant enzyme, 37°C, completely stable | Levilactobacillus brevis |