Cloned (Comment) | Organism |
---|---|
mutants expressed in Escherichia coli DH5alpha cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Q493R/T509A/M532T/N550S/G559S/N566S | mutant showing improved thermostability retaining 75% of its activity when heated at 80°C for 10 min | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.208 | - |
4-nitrophenyl-beta-D-glucuronide | wild type enzyme | Escherichia coli | |
0.414 | - |
4-nitrophenyl-beta-D-glucuronide | mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel chelate affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of the wild type and mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S are approximately 142.9 and 43.1 units/mg at 37°C and pH 6.5 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucuronide + H2O | - |
Escherichia coli | 4-nitrophenol + D-glucuronic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GUS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
wild type enzyme | Escherichia coli |
65 | - |
mutant enzymes Q493R/T509A/M532T/N550S/G559S/N566S | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the wild type enzyme loses its activity completely after 10 min at 70°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
4-nitrophenyl-beta-D-glucuronide | mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S | Escherichia coli | |
1500 | - |
4-nitrophenyl-beta-D-glucuronide | wild type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Escherichia coli |