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Literature summary for 3.2.1.31 extracted from
- Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability (2007), Protein Eng. Des. Sel., 20, 319-325.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutants expressed in Escherichia coli DH5alpha cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q493R/T509A/M532T/N550S/G559S/N566S | mutant showing improved thermostability retaining 75% of its activity when heated at 80°C for 10 min | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.208 | - |
4-nitrophenyl-beta-D-glucuronide | wild type enzyme | Escherichia coli |  |
| 0.414 | - |
4-nitrophenyl-beta-D-glucuronide | mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel chelate affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of the wild type and mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S are approximately 142.9 and 43.1 units/mg at 37°C and pH 6.5 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Escherichia coli | 4-nitrophenol + D-glucuronic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GUS | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
wild type enzyme | Escherichia coli |
| 65 | - |
mutant enzymes Q493R/T509A/M532T/N550S/G559S/N566S | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the wild type enzyme loses its activity completely after 10 min at 70°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1100 | - |
4-nitrophenyl-beta-D-glucuronide | mutant enzyme Q493R/T509A/M532T/N550S/G559S/N566S | Escherichia coli | |
| 1500 | - |
4-nitrophenyl-beta-D-glucuronide | wild type enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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