Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucaric acid 1,4-lactone | 5 mM, 30% inhibition | Aspergillus niger | |
Hg2+ | 1 mM, 37°C, 10 min, 23% inhibition | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
p-nitrophenyl-beta-D-glucuronide | pH 4.6, 37°C | Aspergillus niger | |
0.53 | - |
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal | pH 4.6, 37°C | Aspergillus niger | |
0.71 | - |
beta-GlcA-(1-6)-Gal | pH 4.6, 37°C | Aspergillus niger | |
1.4 | - |
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal | pH 4.6, 37°C | Aspergillus niger | |
1.9 | - |
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal | pH 4.6, 37°C | Aspergillus niger | |
3.6 | - |
4-Me-beta-GlcA-(1-6)-Gal | pH 4.6, 37°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
1 * 68000, SDS-PAGE | Aspergillus niger |
71000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | Pectinex Ultra SP-L, Lot No. G 770 | Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.6 | - |
reaction with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger |
29.3 | - |
reaction with beta-GlcA-(1-6)-Gal | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O | rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger | ? | - |
? | |
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O | rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger | ? | - |
? | |
4-Me-beta-GlcA-(1-6)-Gal + H2O | rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger | ? | - |
? | |
alpha-L-arabinosidase-treated-arabinogalactan-protein + H2O | - |
Aspergillus niger | ? | - |
? | |
beta-GlcA-(1-3)-Gal + H2O | rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger | ? | - |
? | |
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O | rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger | beta-Gal-(1-6)-Gal + beta-D-glucuronic acid | - |
? | |
beta-GlcA-(1-6)-Gal + H2O | rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide | Aspergillus niger | D-galactose + beta-D-glucuronic acid | - |
? | |
additional information | transglycosylation activity | Aspergillus niger | ? | - |
? | |
p-nitrophenyl-beta-D-glucuronide + H2O | - |
Aspergillus niger | p-nitrophenol + beta-D-glucuronic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, SDS-PAGE | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 4.6, 20 min, stable | Aspergillus niger |
75 | - |
pH 4.6, 20 min, complete inactivation | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
reaction with beta-GlcA-(1-6)-Gal and p-nitrophenyl-beta-D-glucuronide | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | 4°C, 24 h, stable | Aspergillus niger |
4.5 | 9 | 37°C, 3 h, stable | Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | isoelectric focusing, pH-range: 3.5-10 | - |
5 |