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Literature summary for 3.2.1.3 extracted from

  • Sakaguchi, M.; Matsushima, Y.; Nankumo, T.; Seino, J.; Miyakawa, S.; Honda, S.; Sugahara, Y.; Oyama, F.; Kawakita, M.
    Glucoamylase of Caulobacter crescentus CB15: Cloning and expression in Escherichia coli and functional identification (2014), AMB Express, 4, 5.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, as fusion protein with Staphylococcus protein A Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
additional information CauloGA gene product that is expressed in Escherichia coli is prone to forming inclusion bodies. Most of the gene product is expressed in a soluble and active form when it was expressed as a fusion protein with Staphylococcus Protein A Caulobacter vibrioides

Inhibitors

Inhibitors Comment Organism Structure
maltoheptaose
-
Caulobacter vibrioides
maltohexaose
-
Caulobacter vibrioides
maltopentaose
-
Caulobacter vibrioides
maltose
-
Caulobacter vibrioides
maltotetraose
-
Caulobacter vibrioides
maltotriose pronounced inhibition above 2 mM Caulobacter vibrioides
additional information enzyme activity is significantly inhibited when the substrate concentration exceeds approximately 10fold the Km value Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
maltopentaose pH 5.0, 40°C Caulobacter vibrioides
0.04
-
maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
0.05
-
maltohexaose pH 5.0, 40°C Caulobacter vibrioides
0.14
-
maltotetraose pH 5.0, 40°C Caulobacter vibrioides
0.16
-
maltotriose pH 5.0, 40°C Caulobacter vibrioides
0.87
-
maltose pH 5.0, 40°C Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A613
-
-
Caulobacter vibrioides CB15 Q9A613
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + 6 H2O
-
Caulobacter vibrioides 7 beta-D-glucose
-
?
maltoheptaose + 6 H2O
-
Caulobacter vibrioides CB15 7 beta-D-glucose
-
?
maltohexaose + 5 H2O
-
Caulobacter vibrioides 6 beta-D-glucose
-
?
maltohexaose + 5 H2O
-
Caulobacter vibrioides CB15 6 beta-D-glucose
-
?
maltopentaose + 4 H2O
-
Caulobacter vibrioides 5 beta-D-glucose
-
?
maltopentaose + 4 H2O
-
Caulobacter vibrioides CB15 5 beta-D-glucose
-
?
maltose + H2O
-
Caulobacter vibrioides 2 beta-D-glucose
-
?
maltotetraose + 3 H2O
-
Caulobacter vibrioides 4 beta-D-glucose
-
?
maltotriose + 2 H2O
-
Caulobacter vibrioides 3 beta-D-glucose
-
?
maltotriose + 2 H2O
-
Caulobacter vibrioides CB15 3 beta-D-glucose
-
?
starch + H2O
-
Caulobacter vibrioides beta-D-glucose + ?
-
?
starch + H2O
-
Caulobacter vibrioides CB15 beta-D-glucose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Caulobacter vibrioides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42.9
-
melting temperature Caulobacter vibrioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.2
-
maltose pH 5.0, 40°C Caulobacter vibrioides
114
-
maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
122
-
maltopentaose pH 5.0, 40°C Caulobacter vibrioides
125
-
maltohexaose pH 5.0, 40°C Caulobacter vibrioides
150
-
maltotriose pH 5.0, 40°C Caulobacter vibrioides
155
-
maltotetraose pH 5.0, 40°C Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Caulobacter vibrioides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.92
-
maltopentaose pH 5.0, 40°C Caulobacter vibrioides
1.05
-
maltohexaose pH 5.0, 40°C Caulobacter vibrioides
1.1
-
maltotetraose pH 5.0, 40°C Caulobacter vibrioides
1.25
-
maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
4.49
-
maltotriose pH 5.0, 40°C Caulobacter vibrioides
38.2
-
maltose pH 5.0, 40°C Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
43.9
-
maltose pH 5.0, 40°C Caulobacter vibrioides
938
-
maltotriose pH 5.0, 40°C Caulobacter vibrioides
1110
-
maltotetraose pH 5.0, 40°C Caulobacter vibrioides
2500
-
maltohexaose pH 5.0, 40°C Caulobacter vibrioides
2850
-
maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
4070
-
maltopentaose pH 5.0, 40°C Caulobacter vibrioides