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Literature summary for 3.2.1.3 extracted from

  • Yoshizaki, Y.; Susuki, T.; Takamine, K.; Tamaki, H.; Ito, K.; Sameshima, Y.
    Characterization of glucoamylase and alpha-amylase from Monascus anka: enhanced production of alpha-amylase in red koji (2010), J. Biosci. Bioeng., 110, 670-674.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
soluble starch + H2O Monascus purpureus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Monascus purpureus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information influence of different culture conditions on enzyme production, overview. A solid barley plate is the most suitable for mycelial growth, but glucoamylase production per growth area does not differ according to the raw material. During enzyme production in red koji, reducing the temperature from 35°C to 25°C for 48 h in the late stages of growth results in 1.4fold higher glucoamylase production with a concomitant increase in protein stability Monascus purpureus
-
mycelium
-
Monascus purpureus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.9
-
purified isozyme 1, pH 5.5, 37°C Monascus purpureus
74.5
-
purified isozyme 2, pH 5.5, 37°C Monascus purpureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
Monascus purpureus ?
-
?

Synonyms

Synonyms Comment Organism
glucoamylase
-
Monascus purpureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Monascus purpureus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Monascus purpureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
isozymes 1 and 2 Monascus purpureus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.1 9.5 purified isozyme 1 Monascus purpureus
2.1 11.4 purified isozyme 2 Monascus purpureus