Application | Comment | Organism |
---|---|---|
biotechnology | enzyme immobilization on polyacrylamide gel results in an enzyme with increases thermostability for use in biocatalysis | Gymnascella citrina |
synthesis | enzyme immobilization on polyacrylamide gel results in an enzyme with increases thermostability for use in biocatalysis | Gymnascella citrina |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilization on polyacrylamide gel highly decreases the entropy and enthalpy of thermal enzyme deactivation | Gymnascella citrina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics of polyacrylamide gel-immobilized purified enzyme | Gymnascella citrina | |
0.031 | - |
starch | immobilized enzyme, pH 5.5, 90°C | Gymnascella citrina | |
0.1 | - |
starch | free enzyme, pH 5.5, 90°C | Gymnascella citrina | |
0.12 | - |
starch | immobilized enzyme, pH 5.5, 70°C | Gymnascella citrina | |
0.18 | - |
starch | free enzyme, pH 5.5, 70°C | Gymnascella citrina | |
0.238 | - |
starch | immobilized enzyme, pH 5.5, 50°C | Gymnascella citrina | |
0.333 | - |
starch | free enzyme, pH 5.5, 50°C | Gymnascella citrina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Gymnascella citrina | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gymnascella citrina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 63.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography to homogeneity | Gymnascella citrina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gymnascella citrina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Gymnascella citrina | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Gymnascella citrina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
free enzyme | Gymnascella citrina |
60 | - |
immobilized enzyme | Gymnascella citrina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | temperature profile of free and immobilized enzyme, overview | Gymnascella citrina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
immobilized enzyme, stable up to, melting temperature of the free enzyme | Gymnascella citrina |
70 | - |
melting temperature of the immobilized enzyme | Gymnascella citrina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
starch | immobilized enzyme, pH 5.5, 50°C | Gymnascella citrina | |
4.12 | - |
starch | free enzyme, pH 5.5, 50°C | Gymnascella citrina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
free enzyme | Gymnascella citrina |
5.5 | - |
immobilized enzyme | Gymnascella citrina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | pH profile of free and immobilized enzyme, overview | Gymnascella citrina |