Cloned (Comment) | Organism |
---|---|
expression of active MGA N-terminal catalytic domain MGAnt, residues 87-955, in COS-1 cells, and overexpression of MGAnt in Drosophila S2 cells, secretion of recombinant proteins | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | a salacinol derivative, inhibition of the isolated recombinant N-terminal catalytic domain | Homo sapiens | |
kotalanol | a salacinol derivative, inhibition of the isolated recombinant N-terminal catalytic domain | Homo sapiens | |
miglitol | a salacinol derivative, inhibition of the isolated recombinant N-terminal catalytic domain | Homo sapiens | |
salacinol | isolated from Salacia reticulata, a plant native to Sri Lanka and India that has been used in the Ayurvedic system of medicine for the treatment of diabetes, inhibition of the isolated recombinant N-terminal catalytic domain | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is anchored in the membrane of small intestinal epithelial cells | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105360 | - |
x * 105360, recombinant N-terminal catalytic domain, MALDI-TOF mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Homo sapiens | the enzyme is responsible for the final step of mammalian starch digestion leading to the release of D-glucose | D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
secreted, recombinant N-terminal catalytic domain from Drosophila S2 cell culture supernatant by chelating resin and anion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelial cell | - |
Homo sapiens | - |
small intestine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl D-glucose | - |
Homo sapiens | 4-nitrophenol + D-glucose | - |
? | |
starch + H2O | - |
Homo sapiens | D-glucose + ? | - |
? | |
starch + H2O | the enzyme is responsible for the final step of mammalian starch digestion leading to the release of D-glucose | Homo sapiens | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 105360, recombinant N-terminal catalytic domain, MALDI-TOF mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
maltase glucoamylase | - |
Homo sapiens |
MGA | - |
Homo sapiens |
More | the enzyme is a family 31 glycoside hydrolase | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00019 | - |
salacinol | pH 6.5, 37°C, recombinant N-terminal catalytic domain | Homo sapiens | |
0.062 | - |
acarbose | pH 6.5 37°C, recombinant N-terminal catalytic domain | Homo sapiens |