Protein Variants | Comment | Organism |
---|---|---|
E400C | cysteinesulfinic acid derivative of C320A/E400C-SO2H has a 700times higher turnover number towards maltose relative to C320A/E400C, while the Km-value is unchanged. Compared to wild-type enzyme, the C400-SO2H derivative has a turnover number of 150-190% and 85-320% on maltooliogosaccharides and isomaltooligosaccharides respectively, while Km-values are similar to that of wild-type for disaccharides and 3.5-5.5fold and 1.8-2.5fold higher for the longer maltooligosaccharides and isomaltooligosaccharides. The inhibition constant of cysteinesulfinic acid derivative of C320A/E400C-SO2H for acarbose increases more than 10000-fold | Aspergillus awamori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | - |
Aspergillus awamori | |
acarbose | - |
Aspergillus awamori | |
beta-O-methylacarviosinide | - |
Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for C320A/E400C and the cysteinesulfinic acid derivative of C320A/E400C | Aspergillus awamori | |
0.1 | - |
maltopentaose | wild-type enzyme | Aspergillus awamori | |
0.11 | - |
maltohexaose | wild-type enzyme | Aspergillus awamori | |
0.12 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
0.18 | - |
maltotetraose | wild-type enzyme | Aspergillus awamori | |
0.29 | - |
maltotriose | wild-type enzyme | Aspergillus awamori | |
3 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
3.4 | - |
4-nitrophenyl alpha-D-glucoside | wild-type enzyme | Aspergillus awamori | |
17 | - |
panose | wild-type enzyme | Aspergillus awamori | |
32 | - |
nigerose | wild-type enzyme | Aspergillus awamori | |
38 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
70 | - |
alpha-D-glucopyranosyl fluoride | wild-type enzyme | Aspergillus awamori | |
150 | - |
kojibiose | wild-type enzyme | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucoside + H2O | - |
Aspergillus awamori | 4-nitrophenol + D-glucose | - |
? | |
alpha-alpha-trehalose + H2O | - |
Aspergillus awamori | alpha-D-glucose | - |
? | |
alpha-D-glucopyranosyl fluoride + H2O | - |
Aspergillus awamori | ? | - |
? | |
amylopectin + H2O | - |
Aspergillus awamori | D-glucose + ? | - |
? | |
glycogen + H2O | - |
Aspergillus awamori | glucose + ? | - |
? | |
isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
kojibiose + H2O | - |
Aspergillus awamori | alpha-D-glucose + D-glucose | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltotriose + H2O | - |
Aspergillus awamori | maltose + glucose | - |
? | |
nigerose + H2O | - |
Aspergillus awamori | 2 alpha-D-glucose | - |
? | |
panose + H2O | - |
Aspergillus awamori | ? | - |
? | |
starch + H2O | soluble | Aspergillus awamori | glucose + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers for C320A/E400C and the cysteinesulfinic acid derivative of C320A/E400C | Aspergillus awamori | |
0.13 | - |
kojibiose | wild-type enzyme | Aspergillus awamori | |
0.14 | - |
nigerose | wild-type enzyme | Aspergillus awamori | |
0.25 | - |
4-nitrophenyl alpha-D-glucoside | wild-type enzyme | Aspergillus awamori | |
0.27 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
6.8 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
6.9 | - |
panose | wild-type enzyme | Aspergillus awamori | |
27.3 | - |
maltopentaose | wild-type enzyme | Aspergillus awamori | |
28 | - |
maltotriose | wild-type enzyme | Aspergillus awamori | |
45 | - |
maltotetraose | wild-type enzyme | Aspergillus awamori | |
49 | - |
maltohexaose | wild-type enzyme | Aspergillus awamori | |
54 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
300 | - |
alpha-D-glucopyranosyl fluoride | wild-type enzyme | Aspergillus awamori |