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Literature summary for 3.2.1.3 extracted from

  • Fierobe, H.P.; Clarke, A.J.; Tull, D.; Svensson, B.
    Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400->Cys of glucoamylase from Aspergillus awamori (1998), Biochemistry, 37, 3753-3759.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E400C cysteinesulfinic acid derivative of C320A/E400C-SO2H has a 700times higher turnover number towards maltose relative to C320A/E400C, while the Km-value is unchanged. Compared to wild-type enzyme, the C400-SO2H derivative has a turnover number of 150-190% and 85-320% on maltooliogosaccharides and isomaltooligosaccharides respectively, while Km-values are similar to that of wild-type for disaccharides and 3.5-5.5fold and 1.8-2.5fold higher for the longer maltooligosaccharides and isomaltooligosaccharides. The inhibition constant of cysteinesulfinic acid derivative of C320A/E400C-SO2H for acarbose increases more than 10000-fold Aspergillus awamori

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin
-
Aspergillus awamori
acarbose
-
Aspergillus awamori
beta-O-methylacarviosinide
-
Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-values for C320A/E400C and the cysteinesulfinic acid derivative of C320A/E400C Aspergillus awamori
0.1
-
maltopentaose wild-type enzyme Aspergillus awamori
0.11
-
maltohexaose wild-type enzyme Aspergillus awamori
0.12
-
maltoheptaose wild-type enzyme Aspergillus awamori
0.18
-
maltotetraose wild-type enzyme Aspergillus awamori
0.29
-
maltotriose wild-type enzyme Aspergillus awamori
3
-
maltose wild-type enzyme Aspergillus awamori
3.4
-
4-nitrophenyl alpha-D-glucoside wild-type enzyme Aspergillus awamori
17
-
panose wild-type enzyme Aspergillus awamori
32
-
nigerose wild-type enzyme Aspergillus awamori
38
-
isomaltose wild-type enzyme Aspergillus awamori
70
-
alpha-D-glucopyranosyl fluoride wild-type enzyme Aspergillus awamori
150
-
kojibiose wild-type enzyme Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucoside + H2O
-
Aspergillus awamori 4-nitrophenol + D-glucose
-
?
alpha-alpha-trehalose + H2O
-
Aspergillus awamori alpha-D-glucose
-
?
alpha-D-glucopyranosyl fluoride + H2O
-
Aspergillus awamori ?
-
?
amylopectin + H2O
-
Aspergillus awamori D-glucose + ?
-
?
glycogen + H2O
-
Aspergillus awamori glucose + ?
-
?
isomaltose + H2O
-
Aspergillus awamori glucose
-
?
kojibiose + H2O
-
Aspergillus awamori alpha-D-glucose + D-glucose
-
?
maltoheptaose + H2O
-
Aspergillus awamori ?
-
?
maltohexaose + H2O
-
Aspergillus awamori ?
-
?
maltopentaose + H2O
-
Aspergillus awamori ?
-
?
maltose + H2O
-
Aspergillus awamori 2 glucose
-
?
maltotetraose + H2O
-
Aspergillus awamori ?
-
?
maltotriose + H2O
-
Aspergillus awamori maltose + glucose
-
?
nigerose + H2O
-
Aspergillus awamori 2 alpha-D-glucose
-
?
panose + H2O
-
Aspergillus awamori ?
-
?
starch + H2O soluble Aspergillus awamori glucose + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information turnover numbers for C320A/E400C and the cysteinesulfinic acid derivative of C320A/E400C Aspergillus awamori
0.13
-
kojibiose wild-type enzyme Aspergillus awamori
0.14
-
nigerose wild-type enzyme Aspergillus awamori
0.25
-
4-nitrophenyl alpha-D-glucoside wild-type enzyme Aspergillus awamori
0.27
-
isomaltose wild-type enzyme Aspergillus awamori
6.8
-
maltose wild-type enzyme Aspergillus awamori
6.9
-
panose wild-type enzyme Aspergillus awamori
27.3
-
maltopentaose wild-type enzyme Aspergillus awamori
28
-
maltotriose wild-type enzyme Aspergillus awamori
45
-
maltotetraose wild-type enzyme Aspergillus awamori
49
-
maltohexaose wild-type enzyme Aspergillus awamori
54
-
maltoheptaose wild-type enzyme Aspergillus awamori
300
-
alpha-D-glucopyranosyl fluoride wild-type enzyme Aspergillus awamori