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Literature summary for 3.2.1.3 extracted from

  • Arica, M.Y.; Alaeddinoglu, N.G.; Hasirci, V.
    Immobilization of glucoamylase onto activated pHEMA/EGDMA microspheres: properties and application to a packed-bed reactor (1998), Enzyme Microb. Technol., 22, 152-157.
No PubMed abstract available
Search for more literature for 3.2.1.3

Application

Application Comment Organism
synthesis the immobilized enzyme with high operational stability can be used for continuous production of glucose from soluble dextrin Aspergillus niger

General Stability

General Stability Organism
high operational stability of the enzyme immobilized onto poly(2-hydroxyethyl methacrylate)/ethylene glycol dimetharylate microspheres Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 Dextrin free enzyme Aspergillus niger
0.83
-
 Dextrin immobilized enzyme Aspergillus niger
6.2
-
 maltose free enzyme Aspergillus niger
27.4
-
 maltose immobilized enzyme Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextrin + H2O
-
 Aspergillus niger ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 free enzyme Aspergillus niger
55
-
 immobilized enzyme Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 30% of maximal activity, immobilized enzyme, 30°C: about 50% of maximal activity, free enzyme, 60°C: about 50% of maximal activity, free enzyme, 60°C: about 90% of maximal activity, immobilized enzyme Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermostability of immobilized glucoamylase increases considerably as a result of covalent immobilization onto poly(2-hydroxyethyl methacrylate)/ethylene glycol dimetharylate microspheres Aspergillus niger
70
-
 250 min, immobilized enzyme loses 60% of its activity, half-life: 220 min. Free enzyme loses 75% of its activity, half-life 140 min Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8 pH 2.5: about 35% of maximal activity, immobilized enzyme, pH 2.5: about 55% of maximal activity, free enzyme, pH 8.0: about 25% of maximal activity, free enzyme, pH 8.0: about 50% of maximal activity, immobilized enzyme Aspergillus niger