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Literature summary for 3.2.1.3 extracted from
- Purification and characterization of alpha-glucosidase complex from the intestine of the frog, Rana japonica (1996), Biochim. Biophys. Acta, 1296, 152-158.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
amylopectin | - |
Rana japonica |  |
| 1.1 | - |
maltose | - |
Rana japonica | |
| 1.4 | - |
glycogen | - |
Rana japonica | |
| 1.7 | - |
starch | soluble | Rana japonica | |
| 1.8 | - |
amylose | - |
Rana japonica | |
| 4 | - |
isomaltose | - |
Rana japonica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 116000 | - |
1 * 116000 + 1 * 212000, SDS-PAGE | Rana japonica |
| 212000 | - |
1 * 116000 + 1 * 212000, SDS-PAGE | Rana japonica |
| 440000 | - |
papain solubilized enzyme, gel filtration | Rana japonica |
| 550000 | - |
detergent solubilized enzyme, gel filtration | Rana japonica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rana japonica | the enzyme is responsible for much of the isomaltase and maltase activities in the intestine of the frog | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rana japonica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rana japonica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | brush border membrane | Rana japonica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Rana japonica | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Rana japonica | glucose + ? | - |
? | |
| glycogen + H2O | - |
Rana japonica | glucose + ? | - |
? | |
| isomaltose + H2O | - |
Rana japonica | glucose | - |
? | |
| maltose + H2O | - |
Rana japonica | 2 glucose | - |
? | |
| additional information | the enzyme is responsible for much of the isomaltase and maltase activities in the intestine of the frog | Rana japonica | ? | - |
? | |
| starch + H2O | soluble | Rana japonica | glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 116000 + 1 * 212000, SDS-PAGE | Rana japonica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | hydrolysis of isomaltose | Rana japonica |
| 6.5 | 7.5 | hydrolysis of soluble starch | Rana japonica |
| 7 | 8 | hydrolysis of maltose | Rana japonica |
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