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Literature summary for 3.2.1.3 extracted from
- Purification and characterization of a glucoamylase from Aspergillus saitoi (1981), J. Biochem., 89, 125-134.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 1 mM, 49% inhibition, glucoamylase M1 | Aspergillus phoenicis |  |
| Mn2+ | - |
Aspergillus phoenicis | |
| Pb2+ | glucoamylase M2 | Aspergillus phoenicis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90000, glucoamylase I, SDS-PAGE | Aspergillus phoenicis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus phoenicis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glucoamylase M1 contains 18% neutral sugar and 0.77% glucosamine | Aspergillus phoenicis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glucoamylase M1 | Aspergillus phoenicis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucoside + H2O | - |
Aspergillus phoenicis | 4-nitrophenol + D-glucose | - |
? | |
| amylopectin + H2O | - |
Aspergillus phoenicis | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
| dextrin + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
| glycogen + H2O | amylose A, n = 25 and amylose B, n = 130 | Aspergillus phoenicis | glucose + ? | - |
? | |
| isomaltose + H2O | - |
Aspergillus phoenicis | glucose | - |
? | |
| maltose + H2O | - |
Aspergillus phoenicis | 2 glucose | - |
? | |
| maltotriose + H2O | - |
Aspergillus phoenicis | maltose + glucose | - |
? | |
| phenyl alpha-maltoside + H2O | - |
Aspergillus phoenicis | glucose + phenyl alpha-glucoside | - |
? | |
| starch + H2O | soluble | Aspergillus phoenicis | glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, glucoamylase I, SDS-PAGE | Aspergillus phoenicis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
glucoamylase M1, stable | Aspergillus phoenicis |
| 50 | - |
pH 6.0, 5 min, stable up to | Aspergillus phoenicis |
| 70 | - |
pH 6.0, 5 min, complete loss of activity | Aspergillus phoenicis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
glucoamylase M1, hydrolysis of soluble starch | Aspergillus phoenicis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
37°C, 1 h, complete loss of activity below | Aspergillus phoenicis |
| 2.5 | 7.5 | 37°C, 1 h, stable | Aspergillus phoenicis |
| 9 | - |
37°C, 1 h, complete loss of activity above | Aspergillus phoenicis |
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